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Research Papers

Investigation of red blood cell antigens with highly fluorescent and stable semiconductor quantum dots

[+] Author Affiliations
Patrícia Maria Albuquerque de Farias, Beate Saegesser Santos, Frederico Duarte de Menezes, Ricardo de Carvalho Ferreira

Federal University of Pernambuco, Recife, Pernambuco, Brazil

Maria Lourdes Barjas-Castro, Vagner Castro, Paulo Roberto Moura Lima

State University of Campinas, Hematology and Transfusion Hemotherapy Center, Campinas, São Paulo, Brazil

Adriana Fontes, Carlos Lenz Cesar

State University of Campinas, Physics Institute Gleb Wataghin, Campinas, São Paulo, Brazil

J. Biomed. Opt. 10(4), 044023 (August 01, 2005). doi:10.1117/1.1993257
History: Received November 08, 2004; Revised March 23, 2005; Accepted March 28, 2005; Published August 01, 2005
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We report a new methodology for red blood cell antigen expression determination by a simple labeling procedure employing luminescent semiconductor quantum dots. Highly luminescent and stable core shell cadmium sulfide/cadmium hydroxide colloidal particles are obtained, with a predominant size of 9nm. The core-shell quantum dots are functionalized with glutaraldehyde and conjugated to a monoclonal anti-A antibody to target antigen-A in red blood cell membranes. Erythrocyte samples of blood groups A+, A2+, and O+ are used for this purpose. Confocal microscopy images show that after 30min of conjugation time, type A+ and A2+ erythrocytes present bright emission, whereas the O+ group cells show no emission. Fluorescence intensity maps show different antigen expressions for the distinct erythrocyte types. The results obtained strongly suggest that this simple labeling procedure may be employed as an efficient tool to investigate quantitatively the distribution and expression of antigens in red blood cell membranes.

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© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Patrícia Maria Albuquerque de Farias ; Beate Saegesser Santos ; Frederico Duarte de Menezes ; Ricardo de Carvalho Ferreira ; Maria Lourdes Barjas-Castro, et al.
"Investigation of red blood cell antigens with highly fluorescent and stable semiconductor quantum dots", J. Biomed. Opt. 10(4), 044023 (August 01, 2005). ; http://dx.doi.org/10.1117/1.1993257


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