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Special Section on Optical Diagnostic Imaging from Bench to Bedside Spectroscopy

Imaging of gene expression in living cells and tissues

[+] Author Affiliations
Robert H. Singer, David S. Lawrence, Ben Ovryn, John Condeelis

Albert Einstein College of Medicine, Department of Anatomy and Structural Biology and Biochemistry, Biophotonics Center, 1300 Morris Park Avenue, Bronx, New York 10461

J. Biomed. Opt. 10(5), 051406 (October 31, 2005). doi:10.1117/1.2103032
History: Received December 16, 2004; Revised April 04, 2005; Accepted April 05, 2005; Published October 31, 2005
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It is possible to observe gene expression within single cells using a tetracycline inducible promoter for activation. Transcription can be observed by using a fluorescent fusion protein to bind nascent RNA. Ultimately, it is desirable to activate a reporter gene within a single cell with only photons. This is achieved by preparing a chemically altered transcription factor that is functionally unable to activate a reporter gene until it is exposed to photon excitation. We apply two-photon imaging to visualize tumor cells expressing a transgene and ultimately this approach will provide the means to activate a specific gene within a single cell within any tissue to ultimately observe its functional significance in situ.

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© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Robert H. Singer ; David S. Lawrence ; Ben Ovryn and John Condeelis
"Imaging of gene expression in living cells and tissues", J. Biomed. Opt. 10(5), 051406 (October 31, 2005). ; http://dx.doi.org/10.1117/1.2103032


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