Special Section on Optical Diagnostic Imaging from Bench to Bedside Coherent Imaging

Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometry

[+] Author Affiliations
Adam Wax, John W. Pyhtila, Robert N. Graf

Duke University, Department of Biomedical Engineering, Durham, North Carolina 27708

Ronald Nines

The Ohio State University, Comprehensive Cancer Center, Columbus, Ohio 43210

Charles W. Boone

National Cancer Institute, Division of Cancer Prevention, Bethesda, MD 20892-7322

Ramachandra R. Dasari, Michael S. Feld

Massachusetts Institute of Technology, G. R. Harrison Spectroscopy Laboratory, Cambridge, Massachusetts 02139-4307

Vernon E. Steele

National Cancer Institute, Division of Cancer Prevention, Bethesda, MD 20892-7322

Gary D. Stoner

The Ohio State University, Comprehensive Cancer Center, Columbus, Ohio 43210

J. Biomed. Opt. 10(5), 051604 (October 14, 2005). doi:10.1117/1.2102767
History: Received December 16, 2004; Revised April 08, 2005; Accepted April 13, 2005; Published October 14, 2005
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Angle-resolved low-coherence interferometry (a/LCI) is used to obtain quantitative, depth-resolved nuclear morphology measurements. We compare the average diameter and texture of cell nuclei in rat esophagus epithelial tissue to grading criteria established in a previous a/LCI study to prospectively grade neoplastic progression. We exploit the depth resolution of a/LCI to exclusively examine the basal layer of the epithelium, approximately 50to100μm beneath the tissue surface, without the need for exogenous contrast agents, tissue sectioning, or fixation. The results of two studies are presented that compare the performance of two a/LCI modalities. Overall, the combined studies show 91% sensitivity and 97% specificity for detecting dysplasia, using histopathology as the standard. In addition, the studies enable the effects of dietary chemopreventive agents, difluoromethylornithine (DFMO) and curcumin, to be assessed by observing modulation in the incidence of neoplastic change. We demonstrate that a/LCI is highly effective for monitoring neoplastic change and can be applied to assessing the efficacy of chemopreventive agents in the rat esophagus.

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© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Adam Wax ; John W. Pyhtila ; Robert N. Graf ; Ronald Nines ; Charles W. Boone, et al.
"Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometry", J. Biomed. Opt. 10(5), 051604 (October 14, 2005). ; http://dx.doi.org/10.1117/1.2102767


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