Research Papers

Whole-body fluorescence lifetime imaging of a tumor-targeted near-infrared molecular probe in mice

[+] Author Affiliations
Sharon Bloch

Washington University School of Medicine, Department of Radiology, St. Louis, Missouri 63110

Frédéric Lesage, Laura McIntosh

ART Advanced Research Technologies Inc., 2300 Alfred-Nobel Boulevard, St-Laurent, Québec, Canada H4S 2A4

Amir Gandjbakhche

National Institutes of Health, National Institute of Child Health and Human Development, Laboratory of Integrative and Medical Biophysics, Bethesda, Maryland 20892

Kexian Liang, Samuel Achilefu

Washington University School of Medicine, Department of Radiology, St. Louis, Missouri 63110

J. Biomed. Opt. 10(5), 054003 (October 04, 2005). doi:10.1117/1.2070148
History: Received April 12, 2005; Revised August 18, 2005; Accepted August 19, 2005; Published October 04, 2005
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Fluorescence lifetime imaging can provide valuable diagnostic information relating to the functional status of diseases. In this study, a near-infrared (NIR) dye-labeled hexapeptide (abbreviated Cyp-GRD) was synthesized. In vitro, Cyp-GRD internalized in nonsmall cell lung cancer cells (A549) without observable cytotoxic or proliferative effects to the cells at a concentration up to 1×104M. Time-domain fluorescence intensity and lifetime imaging of Cyp-GRD injected into A549 tumor-bearing mice revealed that the probe preferentially accumulated in the tumor and the major excretion organs. The fluorescence lifetime of the conjugate at the tumor site was mapped, showing the spatial distribution of the lifetime related to its environment. Additionally, fluorescence intensity image reconstruction obtained by integrating the time-resolved intensities enabled the contrast ratios of tumor-to-kidney or liver in slices at different depths to be displayed. The mean lifetime was 1.03ns for the tumor and 0.80ns for the liver when averaging those pixels exhibiting adequate signal-to-noise ratio, showing the tumor had a higher lifetime average and reflecting the altered physiopathology of the tumor. This study clearly demonstrated the feasibility of whole-body NIR fluorescence lifetime imaging for tumor localization and its spatial functional status in living small animals.

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© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Sharon Bloch ; Frédéric Lesage ; Laura McIntosh ; Amir Gandjbakhche ; Kexian Liang, et al.
"Whole-body fluorescence lifetime imaging of a tumor-targeted near-infrared molecular probe in mice", J. Biomed. Opt. 10(5), 054003 (October 04, 2005). ; http://dx.doi.org/10.1117/1.2070148


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