Research Papers

Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements

[+] Author Affiliations
Michael Gösch, Hans Blom, Sylvain Anderegg, Kerstin Korn, Per Thyberg, Mona Wells, Theo Lasser, Rudolf Rigler

Karolinska Institute, Department of Medical Biochemistry and Biophysics, SE-17177 Stockholm, Sweden and École Polytechnique Fédérale de Lausanne, Laboratoire d’Optique Biomédicale, CH-1015 Lausanne, Switzerland

Anders Magnusson, Sverker Hård

Chalmers University of Technology, Department of Nanoscience and Microtechnology, Photonics Laboratory, SE-412 96 Göteborg, Sweden

J. Biomed. Opt. 10(5), 054008 (October 10, 2005). doi:10.1117/1.2080707
History: Received October 25, 2004; Revised March 25, 2005; Accepted April 29, 2005; Published October 10, 2005
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Dual-color cross-correlation spectroscopy allows the detection and quantification of labeled biomolecules at ultra-low concentrations, whereby the sensitivity of the assay correlates with the measurement time. We now describe a parallel multifocal dual-color spectroscopic configuration employing multiple avalanche photodiodes and hardware correlators. Cross-correlation curves are obtained from several dual-color excitation foci simultaneously. Multifocal dual-color excitation is achieved by splitting each of two laser beams (488 and 633nm) into four sub-beams with the help of two 2×2 fan-out diffractive optical elements (DOEs), and subsequent superposition of the two sets of four foci. The fluorescence emission from double-labeled biomolecules is detected by two 2×2 fiber arrays.

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© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Michael Gösch ; Hans Blom ; Sylvain Anderegg ; Kerstin Korn ; Per Thyberg, et al.
"Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements", J. Biomed. Opt. 10(5), 054008 (October 10, 2005). ; http://dx.doi.org/10.1117/1.2080707


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