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Research Papers

Quality analysis of in vivo near-infrared fluorescence and conventional gamma images acquired using a dual-labeled tumor-targeting probe

[+] Author Affiliations
Jessica P. Houston

Texas A&M University, Photon Migration Laboratory, College Station, Texas 77842-3012

Shi Ke, Wei Wang, Chun Li

University of Texas M.D. Anderson Cancer Center, Department of Experimental Diagnostic Imaging, Box 59, Houston, Texas 77030

Eva M. Sevick-Muraca

Texas A&M University, Photon Migration Laboratory, College Station, Texas 77842-3012

J. Biomed. Opt. 10(5), 054010 (October 31, 2005). doi:10.1117/1.2114748
History: Received November 27, 2004; Revised May 19, 2005; Accepted May 25, 2005; Published October 31, 2005
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The cyclic peptide, cyclopentapeptide cyclo(lys-Arg-Gly-Asp-phe) (c(KRGDf)), which is known to target αvβ3 integrin, is dual-labeled with a radiotracer, indium111, for gamma scintigraphy as well as with a near-infrared dye, IRDye800, for continuous-wave (cw) imaging of αvβ3 positive human M21 melanoma in xenografts. Twenty-four hours after administration of the dual-labeled peptide at a dose equivalent to 90μCi of In111 and 5nmol of near-infrared (NIR) dye, whole-body gamma scintigraphy and cw imaging was conducted. Image acquisition time was 15min for the gamma scintigraphy images and 800ms for the optical images acquired using an NIR sensitive intensified charge-coupled device. The results show that while the target-to-background ratio (TBR) of nuclear and optical imaging were similar for surface regions of interest and consistent with the origin of gamma and NIR radiation from a common targeted peptide, the signal-to-noise ratio (SNR) was significantly higher for optical than nuclear imaging. Furthermore, an analysis of SNR versus contrast showed greater sensitivity of optical over nuclear imaging for the subcutaneous tumor targets. While tomographic reconstructions are necessary to probe TBR, SNR, and contrast for interior tissues, this work demonstrates for the first time the direct comparison of molecular optical and planar nuclear imaging for surface and subsurface cancers.

Figures in this Article
© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Jessica P. Houston ; Shi Ke ; Eva M. Sevick-Muraca ; Wei Wang and Chun Li
"Quality analysis of in vivo near-infrared fluorescence and conventional gamma images acquired using a dual-labeled tumor-targeting probe", J. Biomed. Opt. 10(5), 054010 (October 31, 2005). ; http://dx.doi.org/10.1117/1.2114748


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