Research Papers

In vivo integrated flow image cytometry and lymph/blood vessels dynamic microscopy

[+] Author Affiliations
Ekaterina I. Galanzha

University of Arkansas for Medical Sciences, Philips Classic Laser Laboratories, Little Rock, Arkansas 72205-7199 and Saratov State University, Research-Education Institute of Optics and Biophotonics, Saratov, Russia, 410012

Valery V. Tuchin

Saratov State University, Research-Education Institute of Optics and Biophotonics, Saratov, Russia, 410012

Vladimir P. Zharov

University of Arkansas for Medical Sciences, Philips Classic Laser Laboratories, Little Rock, Arkansas 72205-7199

J. Biomed. Opt. 10(5), 054018 (October 14, 2005). doi:10.1117/1.2060567
History: Received September 21, 2004; Revised May 17, 2005; Accepted May 18, 2005; Published October 14, 2005
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The high spatial resolution (350nm) transmission digital microscopy (TDM) was developed for real time in vivo imaging of microlymphatics of rat mesentery at a single cell level without any contrast agent. The main mesenteric microstructures (lymph-vessel diameter, valve geometry, cells, etc.) and their dynamics (wall motion, valve function, cell velocity, etc.) were monitored with TDM. Depending on structure size, different magnifications were used to image relatively large whole lymphangion (×4 to ×10) as well as to image single cells (×40 to ×100) in lymph and blood flow including estimation of their shape, size, and aggregation state. Various potential applications of the TDM for in vivo studies are discussed, including visualization of circulating cells in lymph and blood flows, studying the kinetics of platelets, leukocyte rolling, as well as imaging absorbing nonfluorescent mesentery structures and leukocytes with a high optical resolution.

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© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Ekaterina I. Galanzha ; Valery V. Tuchin and Vladimir P. Zharov
"In vivo integrated flow image cytometry and lymph/blood vessels dynamic microscopy", J. Biomed. Opt. 10(5), 054018 (October 14, 2005). ; http://dx.doi.org/10.1117/1.2060567


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