JBO Letters

Targeted DNA transfection into the mouse central nervous system using laser-induced stress waves

[+] Author Affiliations
Yasushi Satoh

National Defense Medical College, Department of Biochemistry I, Tokorozawa 359-8513, Japan

Yasunari Kanda

National Defense Medical College, Department of Pharmacology, Tokorozawa 359-8513, Japan

Mitsuhiro Terakawa, Minoru Obara

Keio University, Department of Electronics and Electrical Engineering, Kohoku-ku, Yokohama 223-8522, Japan

Katsushige Mizuno, Yasuhiro Watanabe

National Defense Medical College, Department of Pharmacology, Tokorozawa 359-8513, Japan

Shogo Endo

Okinawa Institute of Science and Technology, Unit for Molecular Neurobiology of Learning and Memory, JST, Gushikawa, 904-2234, Japan

Hidetoshi Ooigawa, Hiroshi Nawashiro

National Defense Medical College, Department of Neurosurgery, Tokorozawa 359-8513, Japan

Shunichi Sato

National Defense Medical College, Division of Biomedical Information Sciences, Tokorozawa 359-8513, Japan

Kunio Takishima

National Defense Medical College, Department of Biochemistry I, Tokorozawa 359-8513, Japan

J. Biomed. Opt. 10(6), 060501 (November 23, 2005). doi:10.1117/1.2128432
History: Received July 20, 2005; Revised August 29, 2005; Accepted September 15, 2005; Published November 23, 2005
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We investigated the feasibility of gene transfer into the mouse central nervous system (CNS) by applying nanosecond pulsed laser-induced stress waves (LISWs). Intraventricular or hippocampal injection of a reporter gene [enhanced green fluorescent protein (EGFP)] followed by application of LISWs showed this method to be efficient in the CNS of newborn and adult mice. Cells expressing EGFP reside at least 3.5mm from the surface of the tissue, while no apparent damage was detected. Additionally, expression of EGFP was limited to the area that was exposed to LISWs. Using this method, the formulation of plasmid DNA by cationic transfer reagent polyethylenimine proved to be effective for improving transfer efficiency into the CNS.

Figures in this Article
© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Yasushi Satoh ; Yasunari Kanda ; Mitsuhiro Terakawa ; Minoru Obara ; Katsushige Mizuno, et al.
"Targeted DNA transfection into the mouse central nervous system using laser-induced stress waves", J. Biomed. Opt. 10(6), 060501 (November 23, 2005). ; http://dx.doi.org/10.1117/1.2128432


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