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JBO Letters

Erythrocyte structure and dynamics quantified by Hilbert phase microscopy

[+] Author Affiliations
Gabriel Popescu

Massachusetts Institute of Technology, George R. Harrison Spectroscopy Laboratory, Cambridge, Massachusetts 02139

Takahiro Ikeda

Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamamatsu, Shizuoka 434-8601 Japan

Catherine A. Best

Harvard Medical School and Massachusetts General Hospital, Department of Pathology, Boston, Massachusetts 02114

Kamran Badizadegan

Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts 02114

Ramachandra R. Dasari, Michael S. Feld

Massachusetts Institute of Technology, George R. Harrison Spectroscopy Laboratory, Cambridge, Massachusetts 02139

J. Biomed. Opt. 10(6), 060503 (August 26, 2005November 01, 2005November 03, 2005December 30, 2005). doi:10.1117/1.2149847
History: Received August 26, 2005; Revised November 01, 2005; Accepted November 03, 2005; Published December 30, 2005
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We present a new quantitative method for investigating red blood cell morphology and dynamics. The instrument integrates quantitative phase microscopy with an inverted microscope, which makes it particularly suitable for the noninvasive assessment of live erythrocytes. In particular, we demonstrate the ability of this approach to quantify noninvasively cell volume and dynamic morphology. The subnanometer path-length sensitivity at the millisecond time scales is exemplified by measuring the hemoglobin flow out of the cell during hemolysis.

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© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Gabriel Popescu ; Takahiro Ikeda ; Catherine A. Best ; Kamran Badizadegan ; Ramachandra R. Dasari, et al.
"Erythrocyte structure and dynamics quantified by Hilbert phase microscopy", J. Biomed. Opt. 10(6), 060503 (August 26, 2005November 01, 2005November 03, 2005December 30, 2005). ; http://dx.doi.org/10.1117/1.2149847


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