Research Papers

Planar fluorescence imaging using normalized data

[+] Author Affiliations
Vasilis Ntziachristos, Gordon Turner, Joshua Dunham, Stephen Windsor, Antoine Soubret, Jorge Ripoll, Helen A. Shih

Massachusetts General Hospital & Harvard Medical School, Center for Molecular Imaging Research, Laboratory for Biooptics and Molecular Imaging, Charlestown, Massachusetts 02129

J. Biomed. Opt. 10(6), 064007 (December 20, 2005). doi:10.1117/1.2136148
History: Received January 11, 2005; Revised July 19, 2005; Accepted July 21, 2005; Published December 20, 2005
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Fluorescence imaging of tissues has gained significant attention in recent years due to the emergence of appropriate reporter technologies that enable noninvasive sensing of molecular function in vivo. Two major approaches have been used so far for fluorescence molecular imaging, i.e., epi-illumination (reflectance) imaging and fluorescence molecular tomography. Transillumination is an alternative approach that has been employed for imaging tissues in the past and could be similarly beneficial for fluorescence molecular imaging. We investigate data normalization schemes in reflectance and transillumination mode and experimentally demonstrate that normalized transillumination offers significant advantages over planar reflectance imaging and over nonnormalized methods. Our observations, based on phantoms and on postmortem and in vivo mouse measurements display image quality improvement, superior depth sensitivity, and improved imaging accuracy over the nonnormalized methods examined. Normalized planar imaging retains implementation simplicity and could be used to improve on standard fluorescence reflectance imaging and as a simplified alternative to the more integrated and accurate tomographic methods.

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© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Vasilis Ntziachristos ; Gordon Turner ; Joshua Dunham ; Stephen Windsor ; Antoine Soubret, et al.
"Planar fluorescence imaging using normalized data", J. Biomed. Opt. 10(6), 064007 (December 20, 2005). ; http://dx.doi.org/10.1117/1.2136148


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