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Research Papers

Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles

[+] Author Affiliations
Cuiping Yao

Key Laboratory of Biomedical Information Engineering of Ministry of Education, China and Xi’an Jiaotong University, Institute of Biomedical Engineering, School of Life Science and Technology, Xianning xi Road 28, Xi’an 710049, China

Ramtin Rahmanzadeh

Research Center Borstel, Parkallee 1-40, D-23845 Borstel, Germany

Elmar Endl

University Bonn, Institute of Molecular Medicine and Experimental Immunology, Sigmund Freud Strasse 25, D-53105 Bonn, Germany

Zhenxi Zhang

Key Laboratory of Biomedical Information Engineering of Ministry of Education, China and Xi’an Jiaotong University, Institute of Biomedical Engineering, School of Life Science and Technology, Xianning xi Road 28, Xi’an 710049, China

Johannes Gerdes

Research Center Borstel, Parkallee 1-40, D-23845 Borstel, Germany

Gereon Hüttmann

University Lübeck, Institute of Biomedical Optics, Peter-Monnik-Weg 4, D-23562 Lübeck, Germany

J. Biomed. Opt. 10(6), 064012 (December 08, 2005). doi:10.1117/1.2137321
History: Received February 03, 2005; Revised August 08, 2005; Accepted August 22, 2005; Published December 08, 2005
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Irradiation of nanoabsorbers with pico- and nanosecond laser pulses could result in thermal effects with a spatial confinement of less than 50nm. Therefore absorbing nanoparticles could be used to create controlled cellular effects. We describe a combination of laser irradiation with nanoparticles, which changes the plasma membrane permeability. We demonstrate that the system enables molecules to penetrate impermeable cell membranes. Laser light at 532nm is used to irradiate conjugates of colloidal gold, which are delivered by antibodies to the plasma membrane of the Hodgkin’s disease cell line L428 and/or the human large-cell anaplastic lymphoma cell line Karpas 299. After irradiation, membrane permeability is evaluated by fluorescence microscopy and flow cytometry using propidium iodide (PI) and fluorescein isothiocyanate (FITC) dextran. The fraction of transiently permeabilized and then resealed cells is affected by the laser parameter, the gold concentration, and the membrane protein of the different cell lines to which the nanoparticles are bound. Furthermore, a dependence on particle size is found for these interactions in the different cell lines. The results suggest that after optimization, this method could be used for gene transfection and gene therapy.

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© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Cuiping Yao ; Ramtin Rahmanzadeh ; Elmar Endl ; Zhenxi Zhang ; Johannes Gerdes, et al.
"Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticles", J. Biomed. Opt. 10(6), 064012 (December 08, 2005). ; http://dx.doi.org/10.1117/1.2137321


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