Research Papers

Fast three-dimensional laser scanning scheme using acousto-optic deflectors

[+] Author Affiliations
Gaddum Duemani Reddy

Rice University, Department of Bioengineering, Houston, Texas 77005

Peter Saggau

Baylor College of Medicine, Department of Neuroscience, Houston, Texas 77030

J. Biomed. Opt. 10(6), 064038 (December 27, 2005). doi:10.1117/1.2141504
History: Received June 06, 2005; Revised August 17, 2005; Accepted August 17, 2005; Published December 27, 2005
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A scheme for fast 3-D laser scanning using acousto-optic deflectors is proposed and demonstrated. By employing counterpropagating acoustic waves that are both chirped and offset in their frequencies, we show that it is possible to simultaneously scan both axially and laterally with frame rates on the order of tens of kilohertz. This scheme was specifically designed for application with multiphoton imaging, particularly of neurons, where it will enable the concurrent monitoring of physiological signals at multiple locations within a microscopic 3-D volume (350×350×200μm). When used for this purpose, we demonstrate how this scheme would also inherently compensate for spatial dispersion when ultrafast laser pulses are used in acousto-optic multiphoton microscopy.

Figures in this Article
© 2005 Society of Photo-Optical Instrumentation Engineers

Citation

Gaddum Duemani Reddy and Peter Saggau
"Fast three-dimensional laser scanning scheme using acousto-optic deflectors", J. Biomed. Opt. 10(6), 064038 (December 27, 2005). ; http://dx.doi.org/10.1117/1.2141504


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