JBO Letters

Two-photon excited autofluorescence imaging of human retinal pigment epithelial cells

[+] Author Affiliations
Meng Han

University of Heidelberg, Faculty of Clinical Medicine, Mannheim Biomedical Engineering Laboratories, and Kirchhoff Institute for Physics, Heidelberg, Germany

Almut Bindewald-Wittich, Frank G. Holz

University of Bonn, Department of Ophthalmology, Bonn, Germany

Guenter Giese

Max-Planck-Institute for Medical Research, Heidelberg, Germany

Markolf H. Niemz

University of Heidelberg, Faculty of Clinical Medicine, Mannheim Biomedical Engineering Laboratories, Heidelberg, Germany

Sarah Snyder, Hui Sun, Jiayi Yu, Michael Agopov, Olivier La Schiazza, Josef F. Bille

University of Heidelberg, Kirchhoff Institute for Physics, Heidelberg, Germany

J. Biomed. Opt. 11(1), 010501 (February 10, 2006). doi:10.1117/1.2171649
History: Received November 04, 2005; Revised December 09, 2005; Revised December 27, 2005; Accepted December 28, 2005; Published February 10, 2006
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Abstract

Degeneration of retinal pigment epithelial (RPE) cells severely impairs the visual function of retina photoreceptors. However, little is known about the events that trigger the death of RPE cells at the subcellular level. Two-photon excited autofluorescence (TPEF) imaging of RPE cells proves to be well suited to investigate both the morphological and the spectral characteristics of the human RPE cells. The dominant fluorophores of autofluorescence derive from lipofuscin (LF) granules that accumulate in the cytoplasm of the RPE cells with increasing age. Spectral TPEF imaging reveals the existence of abnormal LF granules with blue shifted autofluorescence in RPE cells of aging patients and brings new insights into the complicated composition of the LF granules. Based on a proposed two-photon laser scanning ophthalmoscope, TPEF imaging of the living retina may be valuable for diagnostic and pathological studies of age related eye diseases.

Figures in this Article
© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Meng Han ; Almut Bindewald-Wittich ; Frank G. Holz ; Guenter Giese ; Markolf H. Niemz, et al.
"Two-photon excited autofluorescence imaging of human retinal pigment epithelial cells", J. Biomed. Opt. 11(1), 010501 (February 10, 2006). ; http://dx.doi.org/10.1117/1.2171649


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