Research Papers

Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons

[+] Author Affiliations
Alec M. De Grand

Beth Israel Deaconess Medical Center, Division of Hematology/Oncology, Boston, Massachusetts 02215

Stephen J. Lomnes, Deborah S. Lee, Matthew Pietrzykowski

GE Global Research, Niskayuna, New York 12309

Shunsuke Ohnishi

Beth Israel Deaconess Medical Center, Division of Hematology/Oncology Boston, Massachusetts 02215

Timothy G. Morgan

GE Healthcare Biosciences, Boston, Massachusetts 02215

Andrew Gogbashian, Rita G. Laurence

Brigham and Women’s Hospital, Department of Surgery, Boston, Massachusetts 02115

John V. Frangioni

Beth Israel Deaconess Medical Center, Division of Hematology/Oncology and Department of Radiology, Boston, Massachusetts 02215

J. Biomed. Opt. 11(1), 014007 (February 10, 2006). doi:10.1117/1.2170579
History: Received February 14, 2005; Revised August 31, 2005; Accepted October 06, 2005; Published February 10, 2006
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We demonstrate how to construct calibrated, stable, and inexpensive tissue-like phantoms for near-IR (NIR) fluorescence imaging applications. The bulk phantom material is composed of gelatin, intralipid, hemoglobin, and indocyanine green (ICG). Absorbance, scatter, background fluorescence, and texture can be tuned as desired. NIR fluorescent inclusions are comprised of ICG-labeled polystyrene divinylbenzene beads and Pam78-labeled hydroxyapatite crystals. The former mimic tumor masses of controllable size and contrast agent concentration, and the latter mimic microcalcifications in breast cancer. NIR-fluorescent inclusions can be positioned precisely in phantoms, with one or more regions having different optical properties, and their position can be verified independently using microcomputed tomography. We demonstrate how these phantoms can be used to calibrate and compare imaging systems, and to train surgeons to operate under NIR fluorescence image guidance.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Alec M. De Grand ; Stephen J. Lomnes ; Deborah S. Lee ; Matthew Pietrzykowski ; Shunsuke Ohnishi, et al.
"Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons", J. Biomed. Opt. 11(1), 014007 (February 10, 2006). ; http://dx.doi.org/10.1117/1.2170579


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