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Research Papers

Calibration standards for multicenter clinical trials of fluorescence spectroscopy for in vivo diagnosis

[+] Author Affiliations
Sung K. Chang

Massachusetts General Hospital, Wellman Center for Photomedicine, Boston, Massachusetts 02114

E. Neely Atkinson

University of Texas, M. D. Anderson Cancer Center, Department of Biomathematics, 1515 Holcombe Boulevard, Houston, Texas 77030

Dennis Cox

Rice University, Department of Statistics, 6100 South Main, Houston, Texas 77005

Dan Serachitopol, Brian Pikkula

University of Texas, M. D. Anderson Cancer Center, Center for Biomedical Engineering, Houston, Texas 77030

Nena M. Marín

University of Texas, Department of Biomedical Engineering, Austin, Texas 78712

Nicholas MacKinnon, Calum MacAulay

British Columbia Cancer Research Centre, Cancer Imaging Department, 610 West 10th Avenue, Vancouver, British Columbia V5Z 1L3 Canada

Michele Follen

University of Texas, M. D. Anderson Cancer Center, Center for Biomedical Engineering and Department of Gynecologic Oncology, Houston, Texas 77030 and The University of Texas Health Science Center in Houston, Department of Obstetrics, Gynecology, and Reproductive Sciences, Houston, Texas 77030

Rebecca Richards-Kortum

Rice University, Department of Bioengineering, 6100 South Main, Houston, Texas 77005

J. Biomed. Opt. 11(1), 014010 (October 27, 2004October 24, 2005October 26, 2005February 08, 2006). doi:10.1117/1.2166389
History: Received October 27, 2004; Revised October 24, 2005; Accepted October 26, 2005; Published February 08, 2006
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In the context of clinical trials, calibration protocols for optical instruments that ensure measurement accuracy and the ability to carry out meaningful comparisons of data acquired from multiple instruments are required. A series of calibration standards and procedures are presented to assess technical feasibility of optical devices for cervical precancer detection. Measurements of positive and negative standards, and tissue are made with two generations of research grade spectrometers. Calibration accuracy, ability of standards to correct and account for changes in experimental conditions, and device components are analyzed. The relative frequency of measured calibration standards is investigated retrospectively using statistical analysis of trends in instrument performance. Fluorescence measurements of standards and tissue made with completely different spectrometers show good agreement in intensity and lineshape. Frequency of wavelength calibration standards is increased to every 2h to compensate for thermal drifts in grating mount. Variations in illumination energy detected between standards and patient measurements require probe redesign to allow for simultaneous acquisition of illumination power with every patient measurement. The use of frequent and well-characterized standards enables meaningful comparison of data from multiple devices and unambiguous interpretation of experiments among the biomedical optics community.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Topics

Calibration

Citation

Sung K. Chang ; E. Neely Atkinson ; Dennis Cox ; Dan Serachitopol ; Nena M. Marín, et al.
"Calibration standards for multicenter clinical trials of fluorescence spectroscopy for in vivo diagnosis", J. Biomed. Opt. 11(1), 014010 (October 27, 2004October 24, 2005October 26, 2005February 08, 2006). ; http://dx.doi.org/10.1117/1.2166389


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