0
Research Papers

Measurement of dye diffusion in scattering tissue phantoms using dual-wavelength low-coherence interferometry

[+] Author Affiliations
Trude Støren

Norwegian University of Science and Technology, Department of Physics, N-7491 Trondheim, Norway

Arne Røyset

SINTEF Materials and Chemistry, N-7465 Trondheim, Norway

Lars O. Svaasand

Norwegian University of Science and Technology, Department of Electronics and Telecommunications, N-7491 Trondheim, Norway

Tore Lindmo

Norwegian University of Science and Technology, Department of Physics, N-7491 Trondheim, Norway

J. Biomed. Opt. 11(1), 014017 (January 24, 2006). doi:10.1117/1.2159000
History: Received November 26, 2004; Revised September 07, 2005; Accepted September 16, 2005; Published January 24, 2006
Text Size: A A A

We demonstrate low-coherence interferometry (LCI) for dye diffusion measurements in scattering tissue phantoms. The diffusion coefficient of a phthalocyanine dye in 1.5% agar gel containing scattering Intralipid was measured using a dual-wavelength interfero-meter. One wavelength was matched to the absorption peak of the dye at 675nm. The other, 805nm, was not affected by the dye, and was used to correct for varying sample scattering as a function of depth, assuming a constant ratio between scattering at the two wavelengths. The same wavelength dependence of scattering is assumed for the entire sample, but no a priori knowledge about the amount of scattering is needed. The dye diffusion coefficient was estimated by fitting a mathematical model of the interferometer signal to the measured LCI envelope. We compare results obtained using both a constant-scattering and a depth-resolved-scattering approach to determine the sample scattering. The presented method provides robust estimation of the diffusion coefficient when spatial resolution in determining the depth-resolved scattering is varied. Results indicate that the method is valid for samples having continuous spatial variations in the scattering coefficient over lengths as short as the coherence length of the probing light. The method allows in situ characterization of diffusion in scattering media.

Figures in this Article
© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Trude Støren ; Arne Røyset ; Lars O. Svaasand and Tore Lindmo
"Measurement of dye diffusion in scattering tissue phantoms using dual-wavelength low-coherence interferometry", J. Biomed. Opt. 11(1), 014017 (January 24, 2006). ; http://dx.doi.org/10.1117/1.2159000


Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
 
Your Session has timed out. Please sign back in to continue.
Sign In to Access Full Content

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement


Buy this article ($18 for members, $25 for non-members).
Sign In