Research Papers

In vitro gene transfer to mammalian cells by the use of laser-induced stress waves: effects of stress wave parameters, ambient temperature, and cell type

[+] Author Affiliations
Mitsuhiro Terakawa

Keio University, Department of Electronics and Electrical Engineering, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan

Shunichi Sato, Hiroshi Ashida

National Defense Medical College Research Institute, Division of Biomedical Information Sciences, 3-2, Namiki, Tokorozawa, Saitama 359-8513, Japan

Kazuya Aizawa

Self-Defense Forces Central Hospital, 1-2-24, Ikejiri, Setagaya-ku, Tokyo 154-8532, Japan

Maki Uenoyama, Yoshinori Masaki

National Defense Medical College Research Institute, Division of Environmental Medicine, 3-2, Namiki, Tokorozawa, Saitama 359-8513, Japan

Minoru Obara

Keio University, Department of Electronics and Electrical Engineering, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan

J. Biomed. Opt. 11(1), 014026 (January 24, 2006). doi:10.1117/1.2160407
History: Received July 04, 2005; Revised September 05, 2005; Accepted September 16, 2005; Published January 24, 2006
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Laser-mediated gene transfection has received much attention as a new method for targeted gene therapy because of the high spatial controllability of laser energy. We previously demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISWs). In the present study, we investigated the dependence of transfection efficiency on the laser irradiation conditions and hence stress wave conditions in vitro. We measured characteristics of LISWs used for gene transfection. For NIH 3T3 cells, transfection efficiency was evaluated as functions of laser fluence and number of pulses. The effect of ambient temperature was also investigated, and it was found that change in ambient temperature in a specific range resulted in drastic change in transfection efficiency for NIH 3T3 cells. Gene transfection of different types of cell lines were also demonstrated, where cellular heating increased transfection efficiency for nonmalignant cells, while heating decreased transfection efficiency for malignant cells.

Figures in this Article
© 2006 Society of Photo-Optical Instrumentation Engineers

Topics

Lasers

Citation

Mitsuhiro Terakawa ; Shunichi Sato ; Hiroshi Ashida ; Kazuya Aizawa ; Maki Uenoyama, et al.
"In vitro gene transfer to mammalian cells by the use of laser-induced stress waves: effects of stress wave parameters, ambient temperature, and cell type", J. Biomed. Opt. 11(1), 014026 (January 24, 2006). ; http://dx.doi.org/10.1117/1.2160407


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