Research Papers

Evaluation of trapping efficiency of optical tweezers by dielectrophoresis

[+] Author Affiliations
Eirini Papagiakoumou

National Technical University of Athens, Zografou Campus, School of Applied Mathematical and Physical Sciences, Physics Department, 15780 Athens, Greece

Dorel Pietreanu

Carol Davila Medical University, Biophysics Research Group, Bucharest, Romania

Mersini I. Makropoulou

National Technical University of Athens, Zografou Campus, School of Applied Mathematical and Physical Sciences, Physics Department, 15780 Athens, Greece

Eugenia Kovacs

Carol Davila Medical University, Biophysics Research Group, Bucharest, Romania

Alexander A. Serafetinides

National Technical University of Athens, Zografou Campus, School of Applied Mathematical and Physical Sciences, Physics Department, 15780 Athens, Greece

J. Biomed. Opt. 11(1), 014035 (January 31, 2006). doi:10.1117/1.2165176
History: Received March 16, 2005; Revised September 21, 2005; Accepted September 30, 2005; Published January 31, 2006
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A relatively new method for measuring optically induced forces on microparticles and cells, different from the conventional Brownian motion and viscous drag force calibration methods widely used, is introduced. It makes use of the phenomenon of dielectrophoresis for the calibration of optical tweezers through the dielectrophoretic force calculations. A pair of microelectrodes is fabricated by photolithography on a microscope slide and it is connected to a high-frequency generator. The calibration of the optical tweezers setup is performed by the manipulation of polystyrene beads and yeast cells. Calibration diagrams of the transverse forces versus power are deduced for different cell radii and numerical apertures of the objective lenses. The optical system and the related technique provide a fast and easy method for optical tweezers calibration.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Eirini Papagiakoumou ; Dorel Pietreanu ; Mersini I. Makropoulou ; Eugenia Kovacs and Alexander A. Serafetinides
"Evaluation of trapping efficiency of optical tweezers by dielectrophoresis", J. Biomed. Opt. 11(1), 014035 (January 31, 2006). ; http://dx.doi.org/10.1117/1.2165176


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