Research Papers

High-resolution three-dimensional imaging of biofilm development using optical coherence tomography

[+] Author Affiliations
Chuanwu Xi

University of Illinois at Urbana-Champaign, Department of Civil and Environmental Engineering, Biophotonics Imaging Laboratory, Beckman Institute for Advanced Science and Technology, Urbana, Illinois 61801

Daniel Marks, Simon Schlachter, Wei Luo

University of Illinois at Urbana-Champaign, Biophotonics Imaging Laboratory, Beckman Institute for Advanced Science and Technology, Urbana, Illinois 61801

Stephen A. Boppart

University of Illinois at Urbana-Champaign, Biophotonics Imaging Laboratory, Department of Electrical and Computer Engineering, Department of Bioengineering, Department of Internal Medicine, Beckman Institute for Advanced Science and Technology, Urbana, Illinois 61801

J. Biomed. Opt. 11(3), 034001 (June 26, 2006). doi:10.1117/1.2209962
History: Received October 20, 2005; Revised January 04, 2006; Accepted January 09, 2006; Published June 26, 2006
Text Size: A A A

We describe the use of optical coherence tomography (OCT) for high-resolution, real-time imaging of three-dimensional structure and development of a Pseudomonas aeruginosa biofilm in a standard capillary flow-cell model. As the penetration depth of OCT can reach several millimeters in scattering samples, we are able to observe complete biofilm development on all surfaces of a 1mm×1mm flow-cell. We find that biofilm growing at the bottom of the tube has more structural features including voids, outward projections, and microcolonies while the biofilm growing on the top of the tube is relatively flat and contains less structural features. Volume-rendered reconstructions of cross-sectional OCT images also reveal three-dimensional structural information. These three-dimensional OCT images are visually similar to biofilm images obtained with confocal laser scanning microscopy, but are obtained at greater depths. Based on the imaging capabilities of OCT and the biofilm imaging data obtained, OCT has potential to be used as a non-invasive, label-free, real-time, in-situ and/or in-vivo imaging modality for biofilm characterization.

Figures in this Article
© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Chuanwu Xi ; Daniel Marks ; Simon Schlachter ; Wei Luo and Stephen A. Boppart
"High-resolution three-dimensional imaging of biofilm development using optical coherence tomography", J. Biomed. Opt. 11(3), 034001 (June 26, 2006). ; http://dx.doi.org/10.1117/1.2209962


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.