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Research Papers

Determination of optical properties of human blood in the spectral range 250to1100nm using Monte Carlo simulations with hematocrit-dependent effective scattering phase functions

[+] Author Affiliations
Moritz Friebel

Laser- und Medizin- Technologie GmbH, Berlin, Fabeckstr. 60-62, 14195 Berlin, Germany

André Roggan, Gerhard Müller, Martina Meinke

Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, Institut für Medizinische Physik und Lasermedizin, Fabeckstr. 60-62, 14195 Berlin, Germany

J. Biomed. Opt. 11(3), 034021 (May 24, 2006). doi:10.1117/1.2203659
History: Received September 14, 2005; Revised December 21, 2005; Accepted February 21, 2006; Published May 24, 2006
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The absorption coefficient μa, scattering coefficient μs, and anisotropy factor g of diluted and undiluted human blood (hematocrit 0.84 and 42.1%) are determined under flow conditions in the wavelength range 250to1100nm, covering the absorption bands of hemoglobin. These values are obtained by high precision integrating sphere measurements in combination with an optimized inverse Monte Carlo simulation (IMCS). With a new algorithm, appropriate effective phase functions could be evaluated for both blood concentrations using the IMCS. The best results are obtained using the Reynolds-McCormick phase function with the variation factor α=1.2 for hematocrit 0.84%, and α=1.7 for hematocrit 42.1%. The obtained data are compared with the parameters given by the Mie theory. The use of IMCS in combination with selected appropriate effective phase functions make it possible to take into account the nonspherical shape of erythrocytes, the phenomenon of coupled absorption and scattering, and multiple scattering and interference phenomena. It is therefore possible for the first time to obtain reasonable results for the optical behavior of human blood, even at high hematocrit and in high hemoglobin absorption areas. Moreover, the limitations of the Mie theory describing the optical properties of blood can be shown.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Moritz Friebel ; André Roggan ; Gerhard Müller and Martina Meinke
"Determination of optical properties of human blood in the spectral range 250to1100nm using Monte Carlo simulations with hematocrit-dependent effective scattering phase functions", J. Biomed. Opt. 11(3), 034021 (May 24, 2006). ; http://dx.doi.org/10.1117/1.2203659


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