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Research Papers

Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry

[+] Author Affiliations
John W. Pyhtila, Hongwei Ma, Andrew J. Simnick, Ashutosh Chilkoti, Adam Wax

Duke University, Department of Biomedical Engineering, Fitzpatrick Center for Photonics, Durham, North Carolina 27708

J. Biomed. Opt. 11(3), 034022 (June 09, 2006). doi:10.1117/1.2209561
History: Received February 06, 2006; Accepted March 29, 2006; Published June 09, 2006
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Angle-resolved low coherence interferometry (a/LCI) enables depth-resolved measurements of scattered light that can be used to recover subsurface structural information, such as the size of cell nuclei. Measurements of nuclear morphology, however, can be complicated by coherent scattering between adjacent cell nuclei. Previous studies have eliminated this component by applying a window filter to Fourier transformed angular data, based on the justification that the coherent scattering must necessarily occur over length scales greater than the cell size. To fully study this effect, results of experiments designed to test the validity of this approach are now presented. The a/LCI technique is used to examine light scattered by regular cell arrays, created using stamped adhesive micropatterned substrates. By varying the array spacing, it is demonstrated that cell-to-cell correlations have a predictable effect on light scattering distributions. These results are compared to image analysis of fluorescence micrographs of the cell array samples. The a/LCI results show that the impact of coherent scattering on nuclear morphology measurements can be eliminated through data filtering.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

John W. Pyhtila ; Hongwei Ma ; Andrew J. Simnick ; Ashutosh Chilkoti and Adam Wax
"Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry", J. Biomed. Opt. 11(3), 034022 (June 09, 2006). ; http://dx.doi.org/10.1117/1.2209561


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