Special Section on Pioneers in Biomedical Optics: A.J. Welch

Cell disintegration by laser-induced transient microbubbles and its simultaneous monitoring by interferometry

[+] Author Affiliations
Jörg Neumann, Ralf Brinkmann

Medizinisches Laserzentrum Lübeck GmbH, Peter-Monnik-Weg 4, D-23562 Lübeck, Germany

J. Biomed. Opt. 11(4), 041112 (September 06, 2006). doi:10.1117/1.2339815
History: Received September 28, 2005; Revised April 17, 2006; Accepted April 17, 2006; Published September 06, 2006; Online September 06, 2006
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Selective retina treatment (SRT) is a novel short pulsed laser therapy of several retinal diseases associated with a decreased metabolism at the retinal pigment epithelium (RPE). The range of laser pulse energies is small, in which the desired selective RPE disintegration is achieved without adverse effects to the neural retina. Thus, a real-time dosimetry control is required. We investigated a noninvasive interferometric technique able to monitor microbubble formation around the intracellular melanin granula, which is the origin of the desired RPE damage. A porcine ex vivo RPE model was irradiated by single pulses (350ns1.7μs) of a neodymium: yttrium lithium fluoride laser (527nm). The specimen was simultaneously probed by a Michelson interferometer (helium neon-laser: 633nm) and by a hydrophone. Cell viability assays (Calcein-AM) were performed after irradiation. At threshold radiant exposure for cell death (ED50=129±5mJcm2 for 350ns; ED50=180±5mJcm2 for 1.7μs), the interferometric transients changed due to microbubble formation. No major differences in the bubble dynamics were observed between both pulse durations. An algorithm to determine cell death from the interferometric transients showed less than 10% false positive or false negative results for the applied laser expositions compared to the viability assay. Interferometry is a reliable noncontact technique to monitor RPE disintegration and may serve as real-time dosimetry control during SRT.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Jörg Neumann and Ralf Brinkmann
"Cell disintegration by laser-induced transient microbubbles and its simultaneous monitoring by interferometry", J. Biomed. Opt. 11(4), 041112 (September 06, 2006). ; http://dx.doi.org/10.1117/1.2339815


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