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Special Section on Pioneers in Biomedical Optics: A.J. Welch

Dual modality imaging of a novel rat model of ovarian carcinogenesis

[+] Author Affiliations
Elizabeth M. Kanter

The University of Arizona,Division of Biomedical Engineering, Tucson, Arizona 85721

Ross M. Walker

The University of Arizona,Division of Electrical and Computer Engineering, Tucson, Arizona 85721

Samuel L. Marion

The University of Arizona,Department of Physiology, Tucson, Arizona 85721

Molly Brewer

The University of Arizona,Department of Obstetrics and Gynecology, Tucson, Arizona 85721

Patricia B. Hoyer

The University of Arizona,Department of Physiology, Tucson, Arizona 85721

Jennifer K. Barton

The University of Arizona,Division of Biomedical Engineering, Tucson, Arizona 85721 and The University of Arizona,Division of Electrical and Computer Engineering, Tucson, Arizona 85721

J. Biomed. Opt. 11(4), 041123 (July 25, 2006). doi:10.1117/1.2236298
History: Received July 15, 2005; Revised January 20, 2006; Accepted January 22, 2006; Published July 25, 2006; Online July 25, 2006
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Ovarian cancer is the fifth leading cause of cancer death in women, in part because of the limited knowledge about early stage disease. We develop a novel rat model of ovarian cancer and perform a pilot study to examine the harvested ovaries with complementary optical imaging modalities. Rats are exposed to repeated daily dosing (20days) with 4-vinylcyclohexene diepoxide (VCD) to cause early ovarian failure (model for postmenopause), and ovaries are directly exposed to 7,12-dimethylbenz(a)anthracene (DMBA) to cause abnormal ovarian proliferation and neoplasia. Harvested ovaries are examined with optical coherence tomography (OCT) and light-induced fluorescence (LIF) at one, three, and five months post-DMBA treatment. VCD causes complete ovarian follicle depletion within 8months after onset of dosing. DMBA induces abnormal size, cysts, and neoplastic changes. OCT successfully visualizes normal and abnormal structures (e.g., cysts, bursa, follicular remnant degeneration) and the LIF spectra show statistically significant changes in the ratio of average emission intensity at 390:450nm between VCD-treated ovaries and both normal cycling and neoplastic DMBA-treated ovaries. Overall, this pilot study demonstrates the feasibility of both the novel animal model for ovarian cancer and the ability of optical imaging techniques to visualize ovarian function and health.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Topics

Ovary

Citation

Elizabeth M. Kanter ; Ross M. Walker ; Samuel L. Marion ; Molly Brewer ; Patricia B. Hoyer, et al.
"Dual modality imaging of a novel rat model of ovarian carcinogenesis", J. Biomed. Opt. 11(4), 041123 (July 25, 2006). ; http://dx.doi.org/10.1117/1.2236298


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