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Special Section on Pioneers in Biomedical Optics: A.J. Welch

Linear response range characterization and in vivo application of laser speckle imaging of blood flow dynamics

[+] Author Affiliations
Bernard Choi

University of California, Irvine, Beckman Laser Institute and Medical Clinic, 1002 Health Sciences Road East, Irvine, California 92612

Julio C. Ramirez-San-Juan

University of California, Irvine, Beckman Laser Institute and Medical Clinic, 1002 Health Sciences Road East, Irvine, California 92612 and Instituto Nacional de Astrofisica, Optica y Electronica, Puebla, Mexico

Justin Lotfi

University of California, Irvine, Beckman Laser Institute and Medical Clinic and School of Biological Sciences, 1002 Health Sciences Road East, Irvine, California 92612

J. Stuart Nelson

University of California, Irvine, Beckman Laser Institute and Medical Clinic, 1002 Health Sciences Road East, Irvine, California 92612

J. Biomed. Opt. 11(4), 041129 (September 27, 2005February 27, 2006April 05, 2006August 31, 2006August 31, 2006). doi:10.1117/1.2341196
History: Received September 27, 2005; Revised February 27, 2006; Accepted April 05, 2006; Published August 31, 2006; Online August 31, 2006
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Noninvasive blood flow imaging can provide critical information on the state of biological tissue and the efficacy of approaches to treat disease. With laser speckle imaging (LSI), relative changes in blood flow are typically reported, with the assumption that the measured values are on a linear scale. A linear relationship between the measured and actual flow rate values has been suggested. The actual flow rate range, over which this linear relationship is valid, is unknown. Herein we report the linear response range and velocity dynamic range (VDR) of our LSI instrument at two relevant camera integration times. For integration times of 1 and 10ms, the best case VDR was 80 and 60dB, respectively, and the worst case VDR was 20 and 50dB. The best case VDR values were similar to those reported in the literature for optical Doppler tomography. We also demonstrate the potential of LSI for monitoring blood flow dynamics in the rodent dorsal skinfold chamber model. These findings imply that LSI can provide accurate wide-field maps of microvascular blood flow rate dynamics and highlight heterogeneities in flow response to the application of exogenous agents.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Bernard Choi ; Julio C. Ramirez-San-Juan ; Justin Lotfi and J. Stuart Nelson
"Linear response range characterization and in vivo application of laser speckle imaging of blood flow dynamics", J. Biomed. Opt. 11(4), 041129 (September 27, 2005February 27, 2006April 05, 2006August 31, 2006August 31, 2006). ; http://dx.doi.org/10.1117/1.2341196


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