Research Papers

Fluorescence-enhanced optical tomography of a large tissue phantom using point illumination geometries

[+] Author Affiliations
Ranadhir Roy

Baylor College of Medicine, Photon Migration Laboratory, Houston, Texas 77030

Anuradha Godavarty

Florida International University, Department of Biomedical Engineering, Miami, Florida 33174

Eva M. Sevick-Muraca

Baylor College of Medicine, Photon Migration Laboratory, Houston, Texas 77030

J. Biomed. Opt. 11(4), 044007 (August 25, 2006). doi:10.1117/1.2338807
History: Received July 14, 2005; Revised April 06, 2006; Accepted April 07, 2006; Published August 25, 2006; Online August 25, 2006
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We demonstrate fluorescence-enhanced optical imaging of single and multiple fluorescent targets within a large (1081cm3) phantom using frequency-domain photon migration measurements of fluorescence collected at individual points in response to illumination of excitation light at individual points on the boundary. The tissue phantom was filled with a 1% lipid solution with and without 0.01μM Indocyanine Green (ICG) and targets consisted of vials filled with the 1% lipid containing 12.5μM ICG. Measurements were acquired using a modulated intensified CCD imaging system under different experimental conditions. For 3-D image reconstruction, the gradient-based penalty modified barrier function (PMBF) method with simple bounds constrained truncated Newton with trust region method (CONTN) was used. Targets of 0.5, 0.6, and 1.0cm3 at depths of 1.42.8cm from the phantom surface were tomographically reconstructed. This work demonstrates the practicality of fluorescence-enhanced tomography in clinically relevant volumes.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Ranadhir Roy ; Anuradha Godavarty and Eva M. Sevick-Muraca
"Fluorescence-enhanced optical tomography of a large tissue phantom using point illumination geometries", J. Biomed. Opt. 11(4), 044007 (August 25, 2006). ; http://dx.doi.org/10.1117/1.2338807


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