Research Papers

Doppler optical coherence tomography to monitor the effect of photodynamic therapy on tissue morphology and perfusion

[+] Author Affiliations
Maurice C. G. Aalders

University of Amsterdam, Academic Medical Center, Laser Center, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands and The Netherlands Cancer Institute, Department of Experimental Therapy, Antoni van Leeuwenhoek ziekenhuis, Amsterdam, The Netherlands

Martijn Triesscheijn, Marjan Ruevekamp

The Netherlands Cancer Institute, Department of Experimental Therapy, Antoni van Leeuwenhoek ziekenhuis, Amsterdam, The Netherlands

Martijn de Bruin

University of Amsterdam, Academic Medical Center, Laser Center, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands

Paul Baas

The Netherlands Cancer Institute, Department of Thoracic Oncology, Antoni van Leeuwenhoek ziekenhuis, Amsterdam, The Netherlands

Dirk J. Faber

University of Amsterdam, Academic Medical Center, Laser Center, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands

Fiona A. Stewart

The Netherlands Cancer Institute, Department of Experimental Therapy, Antoni van Leeuwenhoek ziekenhuis, Amsterdam, The Netherlands

J. Biomed. Opt. 11(4), 044011 (August 31, 2006). doi:10.1117/1.2337302
History: Received September 02, 2005; Revised March 28, 2006; Accepted April 03, 2006; Published August 31, 2006; Online August 31, 2006
Text Size: A A A

We investigated the feasibility of using optical coherence tomography (OCT) for noninvasive real-time visualization of the vascular effects of photodynamic therapy (PDT) in normal and tumor tissue in mice. Perfusion control measurements were initially performed after administrating vaso-active drugs or clamping of the subcutaneous tumors. Subsequent measurements were made on tumor-bearing mice before and after PDT using the photosensitizer meta-tetrahydroxyphenylchlorin (mTHPC). Tumors were illuminated using either a short drug light interval (D-L, 3h), when mTHPC is primarily located in the tumor vasculature or a long D-L interval (48h), when the drug is distributed throughout the whole tumor. OCT enabled visualization of the different layers of tumor, and overlying skin with a maximal penetration of 0.51mm. PDT with a short D-L interval resulted in a significant decrease of perfusion in the tumor periphery, to 20% of pre-treatment values at 160min, whereas perfusion in the skin initially increased by 10% (at 25min) and subsequently decreased to 60% of pre-treatment values (at 200min). PDT with a long D-L interval did not induce significant changes in perfusion. The concept of using noninvasive OCT measurements for monitoring early, treatment-related changes in morphology and perfusion may have applications in evaluating effects of anti-angiogenic or antivascular (cancer) therapy.

Figures in this Article
© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Maurice C. G. Aalders ; Martijn Triesscheijn ; Marjan Ruevekamp ; Martijn de Bruin ; Paul Baas, et al.
"Doppler optical coherence tomography to monitor the effect of photodynamic therapy on tissue morphology and perfusion", J. Biomed. Opt. 11(4), 044011 (August 31, 2006). ; http://dx.doi.org/10.1117/1.2337302


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.