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Research Papers

Molecular contrast optical coherence tomography: a pump-probe scheme using indocyanine green as a contrast agent

[+] Author Affiliations
Zahid Yaqoob, Emily McDowell, Jigang Wu, Xin Heng, Jeff Fingler, Changhuei Yang

California Institute of Technology, Department of Electrical Engineering, Biophotonics Laboratory, 4 Moore Building, M/C 136-93, 1200 E. California Blvd., Pasadena, California 91125

J. Biomed. Opt. 11(5), 054017 (October 17, 2005May 13, 2006June 20, 2006October 26, 2006October 26, 2006). doi:10.1117/1.2360525
History: Received October 17, 2005; Revised May 13, 2006; Accepted June 20, 2006; Published October 26, 2006; Online October 26, 2006
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The use of indocyanine green (ICG), a U.S. Food and Drug Administration approved dye, in a pump-probe scheme for molecular contrast optical coherence tomography (MCOCT) is proposed and demonstrated for the first time. In the proposed pump-probe scheme, an optical coherence tomography (OCT) scan of the sample containing ICG is first acquired. High fluence illumination (190kJcm2) is then used to permanently photobleach the ICG molecules—resulting in a permanent alteration of the overall absorption of the ICG. A second OCT scan is next acquired. The difference of the two OCT scans is used to determine the depth resolved distribution of ICG within a sample. To characterize the extent of photobleaching in different ICG solutions, we determine the cumulative probability of photobleaching, ϕB,cum, defined as the ratio of the total photobleached ICG molecules to the total photons absorbed by the ground state molecules. An empirical study of ICG photobleaching dynamics shows that ϕB,cum decreases with fluence as well as with increasing dye concentration. The quantity ϕB,cum is useful for estimating the extent of photobleaching in an ICG sample (MCOCT contrast) for a given fluence of the pump illumination. The paper also demonstrates ICG-based MCOCT imaging in tissue phantoms as well as within stage 54 Xenopus laevis.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Zahid Yaqoob ; Emily McDowell ; Jigang Wu ; Xin Heng ; Jeff Fingler, et al.
"Molecular contrast optical coherence tomography: a pump-probe scheme using indocyanine green as a contrast agent", J. Biomed. Opt. 11(5), 054017 (October 17, 2005May 13, 2006June 20, 2006October 26, 2006October 26, 2006). ; http://dx.doi.org/10.1117/1.2360525


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