Research Papers

Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration

[+] Author Affiliations
Frank Dubois, Catherine Yourassowsky, Olivier Monnom, Jean-Claude Legros

Université Libre de Bruxelles, Department of Physical Chemistry, Microgravity Research Center, Faculty of Applied Sciences, Brussels, Belgium 1050

Olivier Debeir, Philippe Van Ham

Université Libre de Bruxelles, Department of Logical and Numerical Systems, Faculty of Applied Sciences, Brussels, Belgium 1050

Robert Kiss, Christine Decaestecker

Université Libre de Bruxelles, Laboratory of Toxicology, Institute of Pharmacy, Brussels, Belgium 1050 and Belgian National Fund for Scientific Research, Brussels, Belgium

J. Biomed. Opt. 11(5), 054032 (September 26, 2006). doi:10.1117/1.2357174
History: Received February 28, 2006; Revised July 06, 2006; Accepted July 21, 2006; Published September 26, 2006; Online September 26, 2006
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Cancer cell motility and invasion are critical targets for anticancer therapeutics. Whereas in vitro models could be designed for rapid screening with a view to investigate these targets, careful consideration must be given to the construction of appropriate model systems. Most investigations focus on two-dimensional (2-D) assays despite the fact that increasing evidence suggests that migration across rigid and planar substrates fails to recapitulate in vivo behavior. In contrast, few systems enable three-dimensional (3-D) cell migration to be quantitatively analyzed. We previously developed a digital holographic microscope (DHM) working in transmission with a partially spatial coherence source. This configuration avoids the noise artifacts of laser illumination and makes possible the direct recording of information on the 3-D structure of samples consisting of multiple objects embedded in scattering media, such as cell cultures in matrix gels. The software driving our DHM system is equipped with a time-lapse ability that enables the 3-D trajectories of living cells to be reconstituted and quantitatively analyzed.

Figures in this Article
© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Frank Dubois ; Catherine Yourassowsky ; Olivier Monnom ; Jean-Claude Legros ; Olivier Debeir, et al.
"Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration", J. Biomed. Opt. 11(5), 054032 (September 26, 2006). ; http://dx.doi.org/10.1117/1.2357174


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