Research Papers

In vivo high-speed imaging of individual cells in fast blood flow

[+] Author Affiliations
Vladimir P. Zharov

University of Arkansas for Medical Sciences, Philips Classic Laser Biomedical Laboratories, Little Rock, Arkansas 72205-7199

Ekaterina I. Galanzha

University of Arkansas for Medical Sciences, Philips Classic Laser Biomedical Laboratories, Little Rock, Arkansas 72205-7199 and Saratov State University, International Institute of Optics and Biophotonics, Saratov, Russia, 410012

Yulian Menyaev

University of Arkansas for Medical Sciences, Philips Classic Laser Biomedical Laboratories, Little Rock, Arkansas 72205-7199

Valery V. Tuchin

Saratov State University, International Institute of Optics and Biophotonics, Saratov, Russia 410012

J. Biomed. Opt. 11(5), 054034 (September 22, 2006). doi:10.1117/1.2355666
History: Received March 20, 2006; Revised June 27, 2006; Accepted July 17, 2006; Published September 22, 2006; Online September 22, 2006
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In vivo, label-free, high-speed (up to 10,000 with the potential for 40,000 frames per second), high-resolution (up to 300nm) real-time continuous imaging with successive framing of circulating individual erythrocytes, leukocytes, and platelets in fast blood flow is developed. This technique, used in an animal model, reveals the extremely high dynamic deformability of erythrocytes in natural flow. Potential applications of this technique are discussed with focus on time-resolved monitoring of the cell deformation dynamics in the native biological environment, which may have diagnostic value for the early diagnosis of diseases.

Figures in this Article
© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Vladimir P. Zharov ; Ekaterina I. Galanzha ; Yulian Menyaev and Valery V. Tuchin
"In vivo high-speed imaging of individual cells in fast blood flow", J. Biomed. Opt. 11(5), 054034 (September 22, 2006). ; http://dx.doi.org/10.1117/1.2355666


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