Research Papers

In vivo ultrahigh-resolution optical coherence tomography of mouse colon with an achromatized endoscope

[+] Author Affiliations
Alexandre R. Tumlinson

University of Arizona, Division of Biomedical Engineering, Tucson, Arizona 85721 and Medical University of Vienna, Center of Biomedical Engineering and Physics, Christian Doppler Laboratory, Vienna, Austria

Boris Považay

Medical University of Vienna, Center of Biomedical Engineering and Physics, Christian Doppler Laboratory, Vienna, Austria

Lida P. Hariri

University of Arizona, Division of Biomedical Engineering, Tucson, Arizona 85721

James McNally

University of Arizona, Optical Sciences Center, Tucson, Arizona 85721

Angelika Unterhuber, Boris Hermann, Harald Sattmann, Wolfgang Drexler

Medical University of Vienna, Center of Biomedical Engineering and Physics, Christian Doppler Laboratory, Vienna, Austria

Jennifer K. Barton

University of Arizona, Division of Biomedical Engineering, Tucson, Arizona 85721 and University of Arizona, Optical Sciences Center, Tucson, Arizona 85721

J. Biomed. Opt. 11(6), 064003 (November 28, 2006). doi:10.1117/1.2399454
History: Received April 06, 2006; Revised August 21, 2006; Accepted August 21, 2006; Published November 28, 2006; Online November 28, 2006
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Endoscopic ultrahigh-resolution optical coherence tomography (OCT) enables collection of minimally invasive cross-sectional images in vivo, which may be used to facilitate rapid development of reliable mouse models of colon disease as well as assess chemopreventive and therapeutic agents. The small physical scale of mouse colon makes light penetration less problematic than in other tissues and high resolution acutely necessary. In our 2-mm diameter endoscopic time domain OCT system, isotropic ultrahigh-resolution is supported by a center wavelength of 800nm and full-width-at-half-maximum bandwidth of 150nm (mode-locked titanium:sapphire laser) combined with 1:1 conjugate imaging of a small core fiber. A pair of KZFSN5/SFPL53 doublets provides excellent color correction to support wide bandwidth throughout the imaging depth. A slight deviation from normal beam exit angle suppresses collection of the strong back reflection at the exit window surface. Our system achieves axial resolution of 3.2μm in air and 4.4-μm lateral spot diameter with 101-dB sensitivity. Microscopic features too small to see in mouse tissue with conventional resolution systems, including colonic crypts, are clearly resolved. Resolution near the cellular level is potentially capable of identifying abnormal crypt formation and dysplastic cellular organization.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Alexandre R. Tumlinson ; Boris Považay ; Lida P. Hariri ; James McNally ; Angelika Unterhuber, et al.
"In vivo ultrahigh-resolution optical coherence tomography of mouse colon with an achromatized endoscope", J. Biomed. Opt. 11(6), 064003 (November 28, 2006). ; http://dx.doi.org/10.1117/1.2399454


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