Research Papers

Evaluation of dermal thermal damage by multiphoton autofluorescence and second-harmonic-generation microscopy

[+] Author Affiliations
Ming-Gu Lin, Tsung-Lin Yang, Cheng-Tien Chiang, Hsien-Ching Kao, Jin-Ning Lee, Wen Lo

National Taiwan University, Department of Physics, Taipei, Taiwan

Shiou-Hwa Jee

National Taiwan University Hospital and National Taiwan University College of Medicine, Department of Dermatology, Taipei, Taiwan

Yang-Fang Chen, Chen-Yuan Dong

National Taiwan University, Department of Physics, Taipei, Taiwan

Sung-Jan Lin

National Taiwan University Hospital and National Taiwan University College of Medicine, Department of Dermatology, Taipei, Taiwan

J. Biomed. Opt. 11(6), 064006 (December 28, 2006). doi:10.1117/1.2405347
History: Received May 18, 2006; Revised August 21, 2006; Accepted August 24, 2006; Published December 28, 2006; Online December 28, 2006
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We attempt to characterize the degree of skin thermal damage by using multiphoton microscopy to characterize dermal thermal damage. Our results show that dermal collagen and elastic fibers display different susceptibility to thermal injury. Morphologically, dermal collagen starts to denature at 60°C while fracture and aggregation of elastic fibers do not occur until 65°C. With increasing temperatures, the structures of both elastic and collagen fibers deteriorate. While second-harmonic-generation (SHG) imaging is helpful in identifying the denaturation temperature of collagen, autofluorescence (AF) imaging can help to identify the structural alternations of tissue at higher temperatures when SHG signals have decayed. We also employ a ratiometric approach based on the AF-to-SHG index of dermis (ASID) to characterize the degree of dermal thermal damage. Use of the ASID index can bypass the difficulty in analyzing inhomogeneous dermal fibers and show that dermal collagen starts to denature at 60°C. Our results suggest that with additional developments, multiphoton microscopy has potential to be developed into an effective in vivo imaging technique to monitor and characterize dermal thermal damage.

Figures in this Article
© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Ming-Gu Lin ; Tsung-Lin Yang ; Cheng-Tien Chiang ; Hsien-Ching Kao ; Jin-Ning Lee, et al.
"Evaluation of dermal thermal damage by multiphoton autofluorescence and second-harmonic-generation microscopy", J. Biomed. Opt. 11(6), 064006 (December 28, 2006). ; http://dx.doi.org/10.1117/1.2405347


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