0
Research Papers

Early spectral changes of cellular malignant transformation using Fourier transform infrared microspectroscopy

[+] Author Affiliations
Evgeny Bogomolny

Ben Gurion University, Department of Physics, Beer-Sheva 84105, Israel

Mahmoud Huleihel, Yelena Suproun

Ben-Gurion University of the Negev, Medical School, Department of Virology and Developmental Genetics, Beer-Sheva 84105, Israel

Ranjit K. Sahu, Shaul Mordechai

Ben Gurion University, Department of Physics, Beer-Sheva 84105, Israel

J. Biomed. Opt. 12(2), 024003 (March 29, 2007). doi:10.1117/1.2717186
History: Received July 25, 2006; Revised December 21, 2006; Accepted January 12, 2007; Published March 29, 2007
Text Size: A A A

Fourier transform infrared microspectroscopy (FTIR-MSP) is potentially a powerful analytical method for identifying the spectral properties of biological activity in cells. The goal of the present research is the implementation of FTIR-MSP to study early spectral changes accompanying malignant transformation of cells. As a model system, cells in culture are infected by the murine sarcoma virus (MuSV), which induces malignant transformation. The spectral measurements are taken at various postinfection time intervals. To follow up systematically the progress of the spectral changes at early stages of cell transformation, it is essential first to determine and validate consistent and significant spectral parameters (biomarkers), which can evidently discriminate between normal and cancerous cells. Early stages of cell transformation are classified by an array of spectral biomarkers utilizing cluster analysis and discriminant classification function techniques. The classifications indicate that the first spectral changes are detectable much earlier than the first morphological signs of cell transformation. Our results point out that the first spectral signs of malignant transformation are observed on the first and third day of postinfection (PI) (for NIH/3T3 and MEF cell cultures, respectively), while the first visible morphological alterations are observed only on the third and seventh day, respectively. These results strongly support the potential of developing FTIR microspectroscopy as a simple, reagent-free method for early detection of malignancy.

Figures in this Article
© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Evgeny Bogomolny ; Mahmoud Huleihel ; Yelena Suproun ; Ranjit K. Sahu and Shaul Mordechai
"Early spectral changes of cellular malignant transformation using Fourier transform infrared microspectroscopy", J. Biomed. Opt. 12(2), 024003 (March 29, 2007). ; http://dx.doi.org/10.1117/1.2717186


Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
 
Your Session has timed out. Please sign back in to continue.
Sign In to Access Full Content

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement


Buy this article ($18 for members, $25 for non-members).
Sign In