Research Papers

Three-dimensional reconstruction of in vivo bioluminescent sources based on multispectral imaging

[+] Author Affiliations
Chaincy Kuo, Olivier Coquoz, Tamara L. Troy, Heng Xu, Brad W. Rice

Caliper Life Sciences, Xenogen Corporation, 2061 Challenger Drive, Alameda, California 94501

J. Biomed. Opt. 12(2), 024007 (April 19, 2007). doi:10.1117/1.2717898
History: Received August 07, 2006; Revised December 08, 2006; Accepted January 11, 2007; Published April 19, 2007
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A new method is described for obtaining a 3-D reconstruction of a bioluminescent light source distribution inside a living animal subject, from multispectral images of the surface light emission acquired on charge-coupled device (CCD) camera. The method uses the 3-D surface topography of the animal, which is obtained from a structured light illumination technique. The forward model of photon transport is based on the diffusion approximation in homogeneous tissue with a local planar boundary approximation for each mesh element, allowing rapid calculation of the forward Green’s function kernel. Absorption and scattering properties of tissue are measured a priori as input to the algorithm. By using multispectral images, 3-D reconstructions of luminescent sources can be derived from images acquired from only a single view. As a demonstration, the reconstruction technique is applied to determine the location and brightness of a source embedded in a homogeneous phantom subject in the shape of a mouse. The technique is then evaluated with real mouse models in which calibrated sources are implanted at known locations within living tissue. Finally, reconstructions are demonstrated in a PC3M-luc (prostate tumor line) metastatic tumor model in nude mice.

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© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Chaincy Kuo ; Olivier Coquoz ; Tamara L. Troy ; Heng Xu and Brad W. Rice
"Three-dimensional reconstruction of in vivo bioluminescent sources based on multispectral imaging", J. Biomed. Opt. 12(2), 024007 (April 19, 2007). ; http://dx.doi.org/10.1117/1.2717898


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