Research Papers

Nonlinear optical interference of two successive coherent anti-Stokes Raman scattering signals for biological imaging applications

[+] Author Affiliations
Eun Seong Lee, Jae Yong Lee, Yong Shim Yoo

Korea Research Institute of Standards and Science, Division of Advanced Technology, P.O. Box 102, Yusung-Taejeon 305-600, South Korea

J. Biomed. Opt. 12(2), 024010 (April 02, 2007). doi:10.1117/1.2718560
History: Received October 12, 2006; Revised December 07, 2006; Accepted December 07, 2006; Published April 02, 2007
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The nonlinear optical interference of two successively generated coherent anti-Stokes Raman scattering (CARS) signals from two different samples placed in series is demonstrated for the imaging performance, in which a collinear phase matching geometry is used. The relative phase of two CARS signals is controlled by a phase-shifting unit made of dispersive glass materials of which the thickness can be precisely varied. The clear interference fringes are observed as the thickness of the phase-shifting unit changes. The interference effect is then utilized to achieve a better quality CARS image of a biological tissue taken from a mouse skin. Placing the tissue in the second sample position and performing raster scans of the laser beams on it, we can acquire a CARS image of higher contrast compared to the normal image obtained without interferometric implementation.

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© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Eun Seong Lee ; Jae Yong Lee and Yong Shim Yoo
"Nonlinear optical interference of two successive coherent anti-Stokes Raman scattering signals for biological imaging applications", J. Biomed. Opt. 12(2), 024010 (April 02, 2007). ; http://dx.doi.org/10.1117/1.2718560


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