Research Papers

Multiphoton fluorescence and second harmonic generation microscopy for imaging infectious keratitis

[+] Author Affiliations
Hsin-Yuan Tan

Chang Gung University,College of Medicine, Chang Gung Memorial Hospital, Department of Ophthalmology, Tao Yuan, Taiwan and National Taiwan University,College of Medicine and College of Engineering, Institute of Biomedical Engineering, Taipei, Taiwan

Yen Sun, Wen Lo, Shu-Wen Teng, Ruei-Jr Wu

National Taiwan University,Department of Physics, Taiepei, Taiwan

Shiou-Hwa Jee

National Taiwan University,College of Medicine, National Taiwan University Hospital, Department of Dermatology, Taipei, Taiwan

Wei-Chou Lin

National Taiwan University Hospital,Department of Pathology, Taipei, Taiwan

Ching-Hsi Hsiao, Hsin-Chiung Lin, Yeong-Fong Chen, David Hui-Kang Ma, Samuel Chao-Ming Huang

Chang Gung University,College of Medicine, Chang Gung Memorial Hospital, Department of Ophthalmology, Tao Yuan, Taiwan

Sung-Jan Lin

National Taiwan University,College of Medicine, National Taiwan University Hospital, Department of Dermatology, Taipei, Taiwan

Chen-Yuan Dong

National Taiwan University,Department of Physics, Taipei, Taiwan

J. Biomed. Opt. 12(2), 024013 (April 24, 2007). doi:10.1117/1.2717133
History: Received June 17, 2006; Revised January 11, 2007; Accepted January 11, 2007; Published April 24, 2007
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The purpose of this study is to demonstrate the application of multiphoton fluorescence and second harmonic generation (SHG) microscopy for the ex-vivo visualization of human corneal morphological alterations due to infectious processes. The structural alterations of both cellular and collagenous components can be respectively demonstrated using fluorescence and SHG imaging. In addition, pathogens with fluorescence may be identified within turbid specimens. Our results show that multiphoton microscopy is effective for identifying structural alterations due to corneal infections without the need of histological processing. With additional developments, multiphoton microscopy has the potential to be developed into an imaging technique effective in the clinical diagnosis and monitoring of corneal infections.

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© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Hsin-Yuan Tan ; Yen Sun ; Wen Lo ; Shu-Wen Teng ; Ruei-Jr Wu, et al.
"Multiphoton fluorescence and second harmonic generation microscopy for imaging infectious keratitis", J. Biomed. Opt. 12(2), 024013 (April 24, 2007). ; http://dx.doi.org/10.1117/1.2717133


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