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Research Papers

Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer

[+] Author Affiliations
Kristen E. Adams

Baylor College of Medicine, Department of Radiology, One Baylor Plaza, MS 360, Houston, Texas 77030 and Rice University, Houston, Texas 77005

Shi Ke, Sunkuk Kwon, Feng Liang

Baylor College of Medicine, Department of Radiology, One Baylor Plaza, MS 360, Houston, Texas 77030

Karen Hirschi

Baylor College of Medicine, Department of Pediatrics, One Baylor Plaza, Houston, Texas 77030

Michel E. Mawad

Baylor College of Medicine, Department of Radiology, One Baylor Plaza, MS 360, Houston, Texas 77030

Michael A. Barry

Baylor College of Medicine, Center for Cell and Gene Therapy, One Baylor Plaza, MS 360, Houston, Texas 77030 and Rice University, Houston, Texas 77005

Eva M. Sevick-Muraca

Baylor College of Medicine, Department of Radiology, One Baylor Plaza, MS 360, Houston, Texas 77030

Zhen Fan, Yang Lu

University of Texas, M.D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030

J. Biomed. Opt. 12(2), 024017 (July 11, 2006December 08, 2006December 11, 2006April 12, 2007). doi:10.1117/1.2717137
History: Received July 11, 2006; Revised December 08, 2006; Accepted December 11, 2006; Published April 12, 2007
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Targeted fluorescent molecular imaging probes may provide an optimal means of detecting disease. Stable, organic fluorophores can be repeatedly excited in vivo by propagated light and consequentially can provide large signal-to-noise ratios (SNRs) for image detection of target tissues. In the literature, many small animal imaging studies are performed with a red excitable dye, Cy5.5, conjugated to the targeting component. We report the comparison of the in vivo fluorescent imaging performance of a near-IR (NIR) and a red-excitable dye. Epidermal growth factor (EGF) was conjugated with Cy5.5 [excitation/emission (ex/em), 660710nm] or IRDye® 800CW (ex/em: 785830nm) for imaging EGF receptor (EGFr) positive (MDA-MB-468) and/or negative (MDA-MB-435) human breast cancer cell lines in subcutaneous xenograft models. The conjugates were injected intravenously at 1-nmol-dye equivalent with and without anti-EGFr monoclonal antibody C225, preadministered 24h prior as a competitive ligand to EGFr. Our images show that while both agents target EGFr, the EGF-IRDye® 800CW evidenced a significantly reduced background and enhanced the tumor-to-background ratio (TBR) compared to the EGF-Cy5.5. Immunohistochemistry shows that EGF causes activation of the EGFr signaling pathway, suggesting that prior to use as a targeting, diagnostic agent, potential deleterious effects should be considered.

Figures in this Article
© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Kristen E. Adams ; Shi Ke ; Karen Hirschi ; Michel E. Mawad ; Michael A. Barry, et al.
"Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer", J. Biomed. Opt. 12(2), 024017 (July 11, 2006December 08, 2006December 11, 2006April 12, 2007). ; http://dx.doi.org/10.1117/1.2717137


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