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Research Papers

Study on the in vitro and in vivo activation of rat hepatic stellate cells by Raman spectroscopy

[+] Author Affiliations
Aiguo Shen

Wuhan University, Institute of Analytical Biomedicine, Wuhan 430072, China and Wuhan University, School of Basic Medicine, Wuhan 430071, China

Zhangxiu Liao, Hui Wang

Wuhan University, School of Basic Medicine, Wuhan 430071, China

Iiho Goan

Wuhan University, Institute of Analytical Biomedicine, Wuhan 430072, China

Yong Wu

Wuhan University, School of Basic Medicine, Wuhan 430071, China

Xiaohua Wang, Zhenyu Yu, Jiming Hu

Wuhan University, Institute of Analytical Biomedicine, Wuhan 430072, China

J. Biomed. Opt. 12(3), 034003 (June 29, 2007). doi:10.1117/1.2749507
History: Received July 31, 2006; Revised January 31, 2007; Accepted February 20, 2007; Published June 29, 2007; October 16, 2007
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The feasibility of a novel and efficient diagnostic method for liver fibrosis using Raman spectroscopy is studied. Confocal Raman spectroscopy (CRS) is utilized to monitor the molecular changes of hepatic stellate cells (HSCs) in vitro as well as in vivo activation. In vitro activation was induced by growth in uncoated plastic plates, while the in vivo activation is induced by a single intraperitoneal injection of carbon tetrachloride (CCl4). The biochemical changes of HSCs during activation such as the loss of retinoid, the increase of α-helical protein, and the increased production of extracellular matrix proteins are observed by CRS. A user-friendly autoclassifying system is also developed to classify Raman spectra of liver injury tissues with a 90% accuracy rate. Raman spectroscopy combined with a fiber optical probe could be potentially accomplished for in vivo detection, which can lead to a novel and efficient diagnosis for liver fibrosis.

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© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Aiguo Shen ; Zhangxiu Liao ; Hui Wang ; Iiho Goan ; Yong Wu, et al.
"Study on the in vitro and in vivo activation of rat hepatic stellate cells by Raman spectroscopy", J. Biomed. Opt. 12(3), 034003 (June 29, 2007). ; http://dx.doi.org/10.1117/1.2749507


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