Research Papers

Angle-resolved Mueller matrix study of light scattering by B-cells at three wavelengths of 442, 633, and 850nm

[+] Author Affiliations
Huafeng Ding, Jun Q. Lu, R. Scott Brock

East Carolina University, Department of Physics, Greenville, North Carolina 27858

Thomas J. McConnell, Jenifer F. Ojeda

East Carolina University, Department of Biology, Greenville, North Carolina 27858

Kenneth M. Jacobs, Xin-Hua Hu

East Carolina University, Department of Physics, Greenville, North Carolina 27858

J. Biomed. Opt. 12(3), 034032 (June 20, 2007). doi:10.1117/1.2749730
History: Received November 27, 2006; Revised January 29, 2007; Accepted February 19, 2007; Published June 20, 2007
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Angle-resolved signals of polarized light scattered by biological cells provide rich information on cell morphology. Quantitative study of these signals can lead to new methods to develop and improve high-throughput instrumentation for cell probing such as scattering-based flow cytometry. We employ a goniometer system with a photoelastic modulation scheme to determine selected Mueller matrix elements of B-cell hydrosol samples. The angular dependence of S11, S12, and S34 is determined from the scattered light signals between 10 and 160deg at the three wavelengths 442, 633, and 850nm. A finite-difference, time-domain (FDTD) method and coated-sphere model are used to investigate the effect of nuclear refractive index on the angle-resolved Mueller elements at different wavelengths using the 3-D structures of selected B cells reconstructed from confocal images. With these results, we demonstrate the value of the light-scattering method in obtaining the cell morphology information.

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© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Huafeng Ding ; Jun Q. Lu ; R. Scott Brock ; Thomas J. McConnell ; Jenifer F. Ojeda, et al.
"Angle-resolved Mueller matrix study of light scattering by B-cells at three wavelengths of 442, 633, and 850nm", J. Biomed. Opt. 12(3), 034032 (June 20, 2007). ; http://dx.doi.org/10.1117/1.2749730


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