Special Section on Optical Coherence Tomography in Ophthalmology

Ultrahigh-resolution imaging of human donor cornea using full-field optical coherence tomography

[+] Author Affiliations
Masahiro Akiba

Yamagata Promotional Organization for Industrial Technology, Yamagata, Japan and TOPCON Advanced Biomedical Imaging Laboratory, Paramus, New Jersey 07652

Naoyuki Maeda, Kazuhiko Yumikake, Takeshi Soma

Osaka University Medical School, Department of Ophthalmology, Suita, Osaka, Japan

Kohji Nishida

Tohoku University Graduate School of Medicine, Department of Ophthalmology and Visual Science, Sendai, Miyagi, Japan

Yasuo Tano

Osaka University Medical School, Department of Ophthalmology, Suita, Osaka, Japan

Kin Pui Chan

Yamagata Promotional Organization for Industrial Technology, Yamagata, Japan and TOPCON Advanced Biomedical Imaging Laboratory, Paramus, New Jersey 07652

J. Biomed. Opt. 12(4), 041202 (July 30, 2007). doi:10.1117/1.2764461
History: Received January 15, 2007; Revised March 11, 2007; Accepted March 22, 2007; Published July 30, 2007
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A feasibility study of ultrahigh-resolution full-field optical coherence tomography (FF-OCT) for a subcellular-level imaging of human donor corneas is presented. The FF-OCT system employed in this experiment is based on a white light interference microscope, where the sample is illuminated by a thermal light source and a horizontal cross-sectional (en face) image is detected using a charge coupled device (CCD) camera. A conventional four-frame phase-shift detection technique is employed to extract the interferometric image from the CCD output. A 95-nm-broadband full-field illumination yields an axial resolution of 2.0μm, and the system covers an area of 850μm×850μm with a transverse resolution of 2.4μm using a 0.3-NA microscope objective and a CCD camera with 512×512 pixels. Starting a measurement from the epithelial to the endothelial side, a series of en face images was obtained. From detected en face images, the epithelial cells, Bowman’s layer, stromal keratocyte, nerve fiber, Descemet’s membrane, and endothelial cell were clearly observed. Keratocyte cytoplasm, its nuclei, and its processes were also separately detected. Two-dimensional interconnectivity of the keratocytes is visualized, and the keratocytes existing between collagen lamellaes are separately extracted by exploiting a high axial resolution ability of FF-OCT.

Figures in this Article
© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Masahiro Akiba ; Naoyuki Maeda ; Kazuhiko Yumikake ; Takeshi Soma ; Kohji Nishida, et al.
"Ultrahigh-resolution imaging of human donor cornea using full-field optical coherence tomography", J. Biomed. Opt. 12(4), 041202 (July 30, 2007). ; http://dx.doi.org/10.1117/1.2764461


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