Research Papers

Investigation of morphometric parameters for granulocytes and lymphocytes as applied to a solution of direct and inverse light-scattering problems

[+] Author Affiliations
Gennady I. Ruban

National Academy of Sciences of Belarus, Stepanov Institute of Physics, Nezavisimosti Avenue 68, Minsk 220072, Belarus

Svetlana M. Kosmacheva, Natalia V. Goncharova

Ministry of Health of Belarus, Centre of Hematology and Transfusiology, Dolginovsky Avenue 160, Minsk 223059, Belarus

Dirk Van Bockstaele

Antwerpen University and Hospital, Wilrijkstraat 10, Edegem B-2650, Belgium

Valery A. Loiko

National Academy of Sciences of Belarus, Stepanov Institute of Physics, Nezavisimosti Avenue 68, Minsk 220072, Belarus

J. Biomed. Opt. 12(4), 044017 (July 05, 2007). doi:10.1117/1.2753466
History: Received June 30, 2006; Revised March 07, 2007; Accepted April 19, 2007; Published July 05, 2007
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Quantitative data on cell structure, shape, and size distribution are obtained by optical measurement of normal peripheral blood granulocytes and lymphocytes in a cell suspension. The cell nuclei are measured in situ. The distribution laws of the cell and nuclei sizes are estimated. The data gained are synthesized to construct morphometric models of a segmented neutrophilic granulocyte and a lymphocyte. Models of interrelation between the cell and nucleus metric characteristics for granulocyte and lymphocyte are obtained. The discovered interrelation decreases the amount of cell-nucleus size combinations that have to be considered under simulation of cell scattering patterns. It allows faster analysis of light scattering to discriminate cells in a real-time scale. Our morphometric data meet the requirements of scanning flow cytometry dealing with the high rate analysis of cells in suspension. Our findings can be used as input parameters for the solution of the direct and inverse light-scattering problems in scanning flow cytometry, dispensing with a costly and time-consuming immunophenotyping of the cells, as well as in turbidimetry and nephelometry. The cell models developed can ensure better interpretations of scattering patterns for an improvement of discriminating capabilities of immunophenotyping-free scanning flow cytometry.

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© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Gennady I. Ruban ; Svetlana M. Kosmacheva ; Natalia V. Goncharova ; Dirk Van Bockstaele and Valery A. Loiko
"Investigation of morphometric parameters for granulocytes and lymphocytes as applied to a solution of direct and inverse light-scattering problems", J. Biomed. Opt. 12(4), 044017 (July 05, 2007). ; http://dx.doi.org/10.1117/1.2753466


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