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Research Papers

Indocyanine-green-embedded PEBBLEs as a contrast agent for photoacoustic imaging

[+] Author Affiliations
Gwangseong Kim

University of Michigan, Department of Chemistry, Ann Arbor, Michigan 48109

Sheng-Wen Huang

University of Michigan, Department of Biomedical Engineering, Ann Arbor, Michigan 48109

Kathleen C. Day

University of Michigan, Department of Urology and Comprehensive Cancer Center, Ann Arbor, Michigan 48109

Matthew O’Donnell

University of Michigan, Department of Biomedical Engineering, Ann Arbor, Michigan 48109

Rodney R. Agayan

University of Michigan, Department of Chemistry, Ann Arbor, Michigan 48109

Mark A. Day

University of Michigan, Department of Urology and Comprehensive Cancer Center, Ann Arbor, Michigan 48109

Raoul Kopelman

University of Michigan, Department of Chemistry, Ann Arbor, Michigan 48109

Shai Ashkenazi

University of Michigan, Department of Biomedical Engineering, Ann Arbor, Michigan 48109

J. Biomed. Opt. 12(4), 044020 (August 27, 2007). doi:10.1117/1.2771530
History: Received November 19, 2006; Revised March 29, 2007; Accepted April 28, 2007; Published August 27, 2007
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Nanoparticles 100nm in diameter containing indocyanine green (ICG) have been developed as a contrast agent for photoacoustic (PA) imaging based on (photonic explorers for biomedical use by biologically localized embedding PEBBLE) technology using organically modified silicate (ormosil) as a matrix. ICG is an FDA-approved dye with strong optical absorption in the near-infrared (NIR) region, where light can penetrate deepest into biological tissue. A photoacoustic imaging system was used to study image contrast as a function of PEBBLE concentration in phantom objects. ICG-embedded ormosil PEBBLEs showed improved stability in aqueous solution compared with free ICG dye. The particles were conjugated with HER-2 antibody for breast cancer and prostate cancer cell targeting. Initial in vitro characterization shows high contrast and high efficiency for binding to prostate cancer cells. ICG can also be used as a photosensitizer (generating toxic oxygen by illumination) for photodynamic therapy. We have measured the photosensitization capability of ICG-embedded ormosil nanoparticles. This feature can be utilized to combine detection and therapeutic functions in a single agent.

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© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Gwangseong Kim ; Sheng-Wen Huang ; Kathleen C. Day ; Matthew O’Donnell ; Rodney R. Agayan, et al.
"Indocyanine-green-embedded PEBBLEs as a contrast agent for photoacoustic imaging", J. Biomed. Opt. 12(4), 044020 (August 27, 2007). ; http://dx.doi.org/10.1117/1.2771530


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