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Research Papers

Label-free molecular imaging of atherosclerotic lesions using multimodal nonlinear optical microscopy

[+] Author Affiliations
Thuc T. Le

Purdue University, Weldon School of Biomedical Engineering, West Lafayette, Indiana 47907

Ingeborg M. Langohr

Purdue University, Department of Comparative Pathobiology, West Lafayette, Indiana 47907

Matthew J. Locker, Michael Sturek

Indiana University, Department of Cellular and Integrative Physiology, Indianapolis, Indiana 46202

Ji-Xin Cheng

Purdue University, Weldon School of Biomedical Engineering, 206 Intramural Drive, West Lafayette, Indiana 47907and Purdue University, Department of Chemistry, West Lafayette, Indiana 47907

J. Biomed. Opt. 12(5), 054007 (October 12, 2007). doi:10.1117/1.2795437
History: Received January 18, 2007; Revised May 10, 2007; Accepted May 14, 2007; Published October 12, 2007
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Arterial tissues collected from Ossabaw swine bearing metabolic syndrome-induced cardiovascular plaques are characterized by multimodal nonlinear optical microscopy that allows coherent anti-Stokes Raman scattering, second-harmonic generation, and two-photon excitation fluorescence imaging on the same platform. Significant components of arterial walls and atherosclerotic lesions, including endothelial cells, extracellular lipid droplets, lipid-rich cells, low-density lipoprotein aggregates, collagen, and elastin are imaged without any labeling. Emission spectra of these components are obtained by nonlinear optical microspectrometry. The nonlinear optical contrast is compared with histology of the same sample. Multimodal nonlinear optical imaging of plaque composition also allows identification of atherosclerotic regions that are vulnerable to rupture risk. The demonstrated capability of nonlinear optical microscopy for label-free molecular imaging of atherosclerotic lesions with 3-D submicrometric resolution suggests its potential application to the diagnosis of atherosclerotic plaques, determination of their rupture risk, and design of individualized drug therapy based on plaque composition.

Figures in this Article
© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Thuc T. Le ; Ingeborg M. Langohr ; Matthew J. Locker ; Michael Sturek and Ji-Xin Cheng
"Label-free molecular imaging of atherosclerotic lesions using multimodal nonlinear optical microscopy", J. Biomed. Opt. 12(5), 054007 (October 12, 2007). ; http://dx.doi.org/10.1117/1.2795437


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