Research Papers

Integral refractive index determination of living suspension cells by multifocus digital holographic phase contrast microscopy

[+] Author Affiliations
Björn Kemper, Sebastian Kosmeier, Patrik Langehanenberg, Gert von Bally

University of Muenster, Laboratory of Biophysics, Robert-Koch-Str. 45, D-48129 Muenster, Germany

Ilona Bredebusch, Wolfram Domschke, Jürgen Schnekenburger

University of Muenster, Department of Medicine B, Albert-Schweitzer-Str. 33, D-48129 Muenster, Germany

J. Biomed. Opt. 12(5), 054009 (October 19, 2007). doi:10.1117/1.2798639
History: Received February 04, 2007; Revised June 09, 2007; Accepted June 11, 2007; Published October 19, 2007
Text Size: A A A

A method for the determination of the integral refractive index of living cells in suspension by digital holographic microscopy is described. Digital holographic phase contrast images of spherical cells in suspension are recorded, and the radius as well as the integral refractive index are determined by fitting the relation between cell thickness and phase distribution to the measured phase data. The algorithm only requires information about the refractive index of the suspension medium and the image scale of the microscope system. The specific digital holographic microscopy advantage of subsequent focus correction allows a simultaneous investigation of cells in different focus planes. Results obtained from human pancreas and liver tumor cells show that the integral cellular refractive index decreases with increasing cell radius.

Figures in this Article
© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Björn Kemper ; Sebastian Kosmeier ; Patrik Langehanenberg ; Gert von Bally ; Ilona Bredebusch, et al.
"Integral refractive index determination of living suspension cells by multifocus digital holographic phase contrast microscopy", J. Biomed. Opt. 12(5), 054009 (October 19, 2007). ; http://dx.doi.org/10.1117/1.2798639


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.