Special Section on Small-Animal Optical Imaging

Three-dimensional optical coherence tomography of whole-muscle autografts as a precursor to morphological assessment of muscular dystrophy in mice

[+] Author Affiliations
Blake R. Klyen, Julian J. Armstrong, Steven G. Adie

The University of Western Australia, School of Electrical, Electronic and Computer Engineering, Optical+Biomedical Engineering Laboratory, 35 Stirling Highway, Crawley, Western Australia 6009, Australia

Hannah G. Radley, Miranda D. Grounds

The University of Western Australia, School of Anatomy and Human Biology, Skeletal Muscle Research Group, 35 Stirling Highway, Crawley, Western Australia 6009, Australia

David D. Sampson

The University of Western Australia, School of Electrical, Electronic and Computer Engineering, Optical+Biomedical Engineering Laboratory, 35 Stirling Highway, Crawley, Western Australia 6009, Australia

J. Biomed. Opt. 13(1), 011003 (February 25, 2008). doi:10.1117/1.2870170
History: Received March 30, 2007; Revised August 30, 2007; Accepted November 16, 2007; Published February 25, 2008
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Three-dimensional optical coherence tomography (3D-OCT) is used to evaluate the structure and pathology of regenerating mouse skeletal muscle autografts for the first time. The death of myofibers with associated inflammation and subsequent new muscle formation in this graft model represents key features of necrosis and inflammation in the human disease Duchenne muscular dystrophy. We perform 3D-OCT imaging of excised autografts and compare OCT images with coregistered histology. The OCT images readily distinguish the necrotic and inflammatory tissue of the graft from the intact healthy muscle fibers in the underlying host tissue. These preliminary findings suggest that, with further development, 3D-OCT could be used as a tool for the evaluation of small-animal muscle morphology and pathology, in particular, for analysis of mouse models of muscular dystrophy.

Figures in this Article
© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Blake R. Klyen ; Julian J. Armstrong ; Steven G. Adie ; Hannah G. Radley ; Miranda D. Grounds, et al.
"Three-dimensional optical coherence tomography of whole-muscle autografts as a precursor to morphological assessment of muscular dystrophy in mice", J. Biomed. Opt. 13(1), 011003 (February 25, 2008). ; http://dx.doi.org/10.1117/1.2870170


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