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Research Papers

Analysis of time-resolved scattering from macroscale bacterial colonies

[+] Author Affiliations
Euiwon Bae

Purdue University, School of Mechanical Engineering, West Lafayette, Indiana 47906

Padmapriya P. Banada, Karleigh Huff, Arun K. Bhunia

Purdue University, Department of Food Science, Molecular Food Microbiology Laboratory, West Lafayette, Indiana 47906

J. Paul Robinson

Purdue University, Bindley Bioscience Center, Purdue University Cytometry Laboratories, Department of Basic Medical Sciences, School of Veterinary Medicine and Weldon School of Biomedical Engineering, West Lafayette, Indiana 47906

E. Daniel Hirleman

Purdue University, School of Mechanical Engineering, West Lafayette, Indiana 47906

J. Biomed. Opt. 13(1), 014010 (January 16, 2008). doi:10.1117/1.2830655
History: Received November 14, 2006; Revised August 28, 2007; Accepted October 26, 2007; Published January 16, 2008
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We investigate the relationship of incubation time and forward-scattering signature for bacterial colonies grown on solid nutrient surfaces. The aim of this research is to understand the colony growth characteristics and the corresponding evolution of the scattering patterns for a variety of pathogenic bacteria relevant to food safety. In particular, we characterized time-varying macroscopic and microscopic morphological properties of the growing colonies and modeled their optical properties in terms of two-dimensional (2-D) amplitude and phase modulation distributions. These distributions, in turn, serve as input to scalar diffraction theory, which is, in turn, used to predict forward-scattering signatures. For the present work, three different species of Listeria were considered: Listeria innocua, Listeria ivanovii, and Listeria monocytogenes. The baseline experiments involved the growth of cultures on brain heart infusion (BHI) agar and the capture of scatter images every 6h over a total incubation period of 42h. The micro- and macroscopic morphologies of the colonies were studied by phase contrast microscopy. Growth curves, represented by colony diameter as a function of time, were compared with the measured time-evolution of the scattering signatures.

Figures in this Article
© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Euiwon Bae ; Padmapriya P. Banada ; Karleigh Huff ; Arun K. Bhunia ; J. Paul Robinson, et al.
"Analysis of time-resolved scattering from macroscale bacterial colonies", J. Biomed. Opt. 13(1), 014010 (January 16, 2008). ; http://dx.doi.org/10.1117/1.2830655


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