Special Section on Optical Clearing of Tissues and Cells

Penetration kinetics of dimethyl sulphoxide and glycerol in dynamic optical clearing of porcine skin tissue in vitro studied by Fourier transform infrared spectroscopic imaging

[+] Author Affiliations
Jingying Jiang

Tianjin University, State Key Laboratory of Precision Measuring Technology and Instruments, Tianjin 300072, China and Tianjin University, Department of Biomedical Engineering, College of Precision Instruments and Opto-Electronics, Tianjin 300072, China

Matthias Boese

Bruker Optik GmbH, Biomedical Products, D-76275 Ettlingen, Germany

Paul Turner

Bruker Optics Limited, Coventry CV4 9GH, England

Ruikang K. Wang

Oregon Health and Science University, Department of Biomedical Engineering, Beaverton, Oregon 97006

J. Biomed. Opt. 13(2), 021105 (March 26, 2008). doi:10.1117/1.2899153
History: Received June 20, 2007; Revised November 06, 2007; Accepted November 22, 2007; Published March 26, 2008
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By use of a Fourier transform infrared (FTIR) spectroscopic imaging technique, we examine the dynamic optical clearing processes occurring in hyperosmotically biocompatible agents penetrating into skin tissue in vitro. The sequential collection of images in a time series provides an opportunity to assess penetration kinetics of dimethyl sulphoxide (DMSO) and glycerol beneath the surface of skin tissue over time. From 2-D IR spectroscopic images and 3-D false color diagrams, we show that glycerol takes at least 30min to finally penetrate the layer of epidermis, while DMSO can be detected in epidermis after only 4min of being topically applied over stratum corneum sides of porcine skin. The results demonstrate the potential of a FTIR spectroscopic imaging technique as an analytical tool for the study of dynamic optical clearing effects when the bio-tissue is impregnated by hyperosmotically biocompatible agents such as glycerol and DMSO.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Jingying Jiang ; Matthias Boese ; Paul Turner and Ruikang K. Wang
"Penetration kinetics of dimethyl sulphoxide and glycerol in dynamic optical clearing of porcine skin tissue in vitro studied by Fourier transform infrared spectroscopic imaging", J. Biomed. Opt. 13(2), 021105 (March 26, 2008). ; http://dx.doi.org/10.1117/1.2899153


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