Special Section on Optical Clearing of Tissues and Cells

Optical clearing of flowing blood using dextrans with spectral domain optical coherence tomography

[+] Author Affiliations
Xiangqun Xu

Zhejiang Sci-Tech University, School of Science, Hangzhou 310018, China

Lingfeng Yu, Zhongping Chen

University of California, Beckman Laser Institute, Irvine, California 92612

J. Biomed. Opt. 13(2), 021107 (April 29, 2008). doi:10.1117/1.2909673
History: Received December 05, 2007; Revised March 04, 2008; Accepted March 05, 2008; Published April 29, 2008
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Spectral domain optical coherence tomography (SDOCT) images have been used to investigate the mechanism of optical clearing in flowing blood using dextrans. The depth reflectivity profiles from SDOCT indicate that dextrans become increasingly more effective in reducing scattering in flowing blood, except for 5mgdl1 of Dx500, with increasing molecular weights (MW 70,000 and 500,000) and concentrations (0.6, 2, and 5mgdl1). Among the tested dextrans, Dx500 at 2mgdl1 had the most significant effect on light scattering reduction with the strongest capability to induce erythrocyte aggregation. Dx500 at 5mgdl1 contributes more refractive index matching but induces a decrease in aggregation that leads to the same level as 0.6mgdl1 Dx500. Previous studies identified various mechanisms of light scattering reduction in stationary blood induced by optical clearing agents. Our results suggest that erythrocyte aggregation is a more important mechanism for optical clearing in flowing blood using dextrans, providing a rational design basis for effective flowing blood optical clearing, which is essential for improving OCT imaging capability through flowing blood.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Xiangqun Xu ; Lingfeng Yu and Zhongping Chen
"Optical clearing of flowing blood using dextrans with spectral domain optical coherence tomography", J. Biomed. Opt. 13(2), 021107 (April 29, 2008). ; http://dx.doi.org/10.1117/1.2909673


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