Special Section on Optical Clearing of Tissues and Cells

Optical clearing in transcutaneous Raman spectroscopy of murine cortical bone tissue

[+] Author Affiliations
Matthew V. Schulmerich, Jacqueline H. Cole, Kathryn A. Dooley, Michael D. Morris

University of Michigan, Department of Chemistry, Ann Arbor, Michigan 48109

Jaclynn M. Kreider, Steven A. Goldstein

University of Michigan Medical School, Department of Orthopaedic Surgery, Orthopaedic Research Laboratory, Ann Arbor, Michigan 48109

J. Biomed. Opt. 13(2), 021108 (March 21, 2008). doi:10.1117/1.2892687
History: Received May 31, 2007; Revised August 13, 2007; Accepted August 24, 2007; Published March 21, 2008
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The effect of optical clearing with glycerol on the Raman spectra of bone tissue acquired transcutaneously on right and left tibiae from four mice is studied. Multiple transcutaneous measurements are obtained from each limb; glycerol is then applied as an optical clearing agent, and additional transcutaneous measurements are taken. Glycerol reduces the noise in the raw spectra (p=0.0037) and significantly improves the cross-correlation between the recovered bone factor and the exposed bone measurement in a low signal-to-noise region of the bone spectra (p=0.0245).

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Matthew V. Schulmerich ; Jacqueline H. Cole ; Kathryn A. Dooley ; Michael D. Morris ; Jaclynn M. Kreider, et al.
"Optical clearing in transcutaneous Raman spectroscopy of murine cortical bone tissue", J. Biomed. Opt. 13(2), 021108 (March 21, 2008). ; http://dx.doi.org/10.1117/1.2892687


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