Special Section on Optical Clearing of Tissues and Cells

Detecting glucose-induced changes in in vitro and in vivo experiments with optical coherence tomography

[+] Author Affiliations
Matti Kinnunen, Risto Myllylä

University of Oulu, Optoelectronics and Measurement Techniques Laboratory and Infotech Oulu, Department of Electrical and Information Engineering, P.O. Box 4500, FIN-90014 Oulu, Finland

Seppo Vainio

University of Oulu, Biocenter Oulu, Laboratory of Developmental Biology, Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, P.O. Box 5000, FIN-90014 Oulu, Finland

J. Biomed. Opt. 13(2), 021111 (April 14, 2008). doi:10.1117/1.2904957
History: Received March 01, 2007; Revised August 20, 2007; Accepted August 24, 2007; Published April 14, 2008
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Optical clearing is a well-known phenomenon. It is based on the matching of refractive indices of a bulk material and scattering particles. The same principle is also used in scattering-based optical measurements of different constituents, such as glucose. By registering changes in scattering, it is possible to evaluate changes in the concentration of a solvent. This work describes the use of optical coherence tomography (OCT) to monitor glucose-induced changes in the optical properties of samples. Intralipid, mouse skin tissue, and mice (C57BL) are used as samples. Differences between in vitro and in vivo measurement conditions, the effect of glucose on the samples’ optical properties, as well as possible problems in OCT experiments are discussed. A comparison of OCT signals from mouse skin samples and mice in vivo shows that the intensity of backscattered radiation is stronger in a living animal than in cultured tissue. Moreover, the effect of glucose on the scattering properties is larger in an in vivo case than in an in vitro case. In comparison with tissue, the effect of glucose is the smallest in Intralipid. The results increase the value of using cultured tissue in developing optical sensing techniques.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Matti Kinnunen ; Risto Myllylä and Seppo Vainio
"Detecting glucose-induced changes in in vitro and in vivo experiments with optical coherence tomography", J. Biomed. Opt. 13(2), 021111 (April 14, 2008). ; http://dx.doi.org/10.1117/1.2904957


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