Special Section on Optical Clearing of Tissues and Cells

Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography

[+] Author Affiliations
Mark Oldham, Harshad Sakhalkar

Duke University Medical Center, Department of Radiation Oncology, Durham, North Carolina 27710

Tim Oliver

Duke University Medical Center, Department of Cell Biology, Durham, North Carolina 27710

G. Allan Johnson

Duke University Medical Center, Department of Radiology, Durham, North Carolina 27710

Mark Dewhirst

Duke University Medical Center, Department of Radiation Oncology, Durham, North Carolina 27710

J. Biomed. Opt. 13(2), 021113 (April 29, 2008). doi:10.1117/1.2907968
History: Received July 27, 2007; Revised November 15, 2007; Accepted December 14, 2007; Published April 29, 2008
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Optical computed tomography (optical-CT) and optical emission computed tomography (optical-ECT) are new techniques that enable unprecedented high-resolution 3-D multimodal imaging of tissue structure and function. Applications include imaging macroscopic gene expression and microvasculature structure in unsectioned biological specimens up to 8cm3. A key requisite for these imaging techniques is effective sample preparation including optical clearing, which enables light transport through the sample while preserving the signal (either light absorbing stain or fluorescent proteins) in representative form. We review recent developments in optical-CT and optical-ECT, and compatible “fluorescence-friendly” optical clearing protocols.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Mark Oldham ; Harshad Sakhalkar ; Tim Oliver ; G. Allan Johnson and Mark Dewhirst
"Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography", J. Biomed. Opt. 13(2), 021113 (April 29, 2008). ; http://dx.doi.org/10.1117/1.2907968


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